5 la ia AR 
313 
of the disease it passed through an evolution similar to that of the 
eruption of variola vera in man. 
The constitutional reaction was very marked, the animals showing 
anorexia and weakness during the active stage of the disease. This 
marked constitutional reaction may be, in part, explained by the intensity 
of the pyogenic process in the operation wound. The temperature re- 
action was similar to that seen after skin inoculation, showing a marked 
rise on the sixth or seventh day. 
The histological examination demonstrates that the lesion at the site 
of inoculation and the lesion of the exanthem on the skin and the mucous 
membranes was variolus in type. In 1 animal focal lesions were demon- 
strated in the seminal vesicles and they were shown to be specific. 
The method of inoculation used in this series of experiments was 
abandoned, as it was impossible to exclude a variolous infection of the 
operation wound. Obviously if this were to take place the experiments 
would be merely repetitions of the skin inoculation complicated by a 
coincident infection of the trachea. 
(6) Inoculation of the epithelium of the trachea, without tracheotomy, 
with the contents of the variola vesicle-—These experiments were devised 
to overcome the difficulty experienced in the previous series resulting 
from infection of the tracheotomy wound. This series consists of exper- 
iments on 10 monkeys (J. cynomologus), of which 1 will be described in 
detail. Six animals were killed at various times after the inoculation to 
obtain material for histological study. 
No. 339. Adult male, VM. cynomologus. Monkey anesthetized with chloroform 
and a large glass tube, having a fire-burnished end, introduced into the larynx 
through the mouth. By way of this tube instruments were introduced to serateh 
the wall of the trachea and to inoculate it with the virus. Variola virus No. 328 
was employed. 
On the seventh day of the disease the body temperature rose to 41° C., and a 
pink, paular elevation 1 millimeter in diameter appeared on the scrotum. On the 
next day small vesicles, surrounded by a bright-red areola, were present upon the 
face, scrotum, hands, and feet. On the following day, the ninth of the experiment, 
new eruptive lesions were found on the face, the scrotum, and the palms and soles. 
Animal chloreformed and autopsied at once. Skin lesions as described above. 
The mucous membrane of the trachea is congested throughout and presents several 
opaque spots on which are minute granular elevations. The trachea and large 
bronchi contain much slimy mucus. The left lung presents an area of consolida- 
tion, 5 millimeters in diameter, about the primary bronchus. On section, this 
area is reddish-brown in color and finely granular. Other organs appear normal. 
Histological examination.—The microscopic study of the organs from these 
monkeys yielded data upon the variolous lesion in situations not previously de- 
scribed. The following descriptions are selected as types of the lesions found: 
No. 345. Trachea.—Lesion of 3 days’ duration. The basement membrane is 
intact. There is no reaction in the submucous tissue. The epithelial cells in a 
small area, which can be included in a single field of the oil immersion lens, con- 
tain various stages of the cytoplasmic forms of Cytoryctes variole. Aside from 
the vacuole about the parasite these cells appear normal. 
