a ee A LW, Be tetas Aen ei Oe Oe ee kl A 
315 
and a variolous pneumonia was demonstrable. No focal lesions were 
found in the bone marrow or testicle. 
A series of five Java monkeys (M. nemistrinus) was inoculated in the 
same manner and with the same virus. The results of these experiments 
were unsatisfactory. No exanthem or constitutional reaction was ob- 
served and we were unable to demonstrate a specific lesion at the site of 
inoculation. 
(c) Inoculation of the lung by inhalation of a spray of the contents of 
the variola vesicle-——These experiments were performed to approximate 
the conditions under which natural infection with smallpox in man 
might be supposed to occur. Of course, the dose of virus is much larger 
than it possibly could be under natural conditions, but at least this 
method approaches more nearly epidemic conditions in that no deliberate 
trauma to an epithelial surface precedes the distribution or application 
of the contagium. Five animals were employed in this series, of which 
the following is. selected to be described in detail: 
No. 207. The monkey’s mouth was held open and a fine spray of vesicle con- 
tents (virus No. 199) was thrown into the throat from an atomizer. The animal 
was observed to breath while the spray was acting. 
No distinct temperature reaction was noted during the 17 days during which 
the animal was kept under observation. No lesion was observed to develop on the 
visible mucous membranes. On the seventh day of the disease a papule appeared 
on the inner aspect of the right arm, at the bend of the elbow. This lesion in- 
creased in size, became vesicular, and on the tenth day was distinctly umbilicated. 
The contents of this lesion was used to inoculate a fresh monkey upon the skin 
of the abdomen. A typical primary lesion developed and was followed by a 
profuse general exanthem. 
Summary.—One of the animals of this series was killed 5 days after 
inoculation. Inspection of the mucous membrane showed no evidence of 
a specific lesion. Sections from various parts of the lungs and the 
trachea were studied microscopically, but no lesions were found. ‘The 
4 animals remaining were under observation for 16 days. In one of them 
a single eruptive lesion appeared on the seventh day of the experiment. 
This lesion was shown to be specific by inoculation of its contents upon 
the skin of a fresh monkey. 
No constitutional reaction or rise of temperature was observed in this 
series of monkeys. 
(d) Inoculation of the lung by inhalation of dry variola virus.—These 
experiments were devised to determine if variola vera could be produced 
in the monkey by employing the contagious material in a dry condition. 
T'wo series of experiments were performed, in one dried pustule contents 
was used and in the other a powder prepared from desiccated crusts or 
disks of a case of variola vera. 
A preliminary experiment was performed to determine how far a 
powder blown into the larynx would enter the lung. A large monkey 
was anesthetized with chloroform and a glass tube containing a mixture 
