316 
of lycopodium and methylene blue powder was introduced into the 
larynx. During an inspiration the powder was forcibly blown into the 
lungs. The animal was killed at once and, on dissection, the smallest 
bronchi which could be made out with the naked eye were found to be 
stained distinctly blue. Histological examination of the lung showed 
lycopodium spores in the bronchi and the alveoh. 
No. 160. A monkey was put under chloroform anesthesia and the same _pro- 
cedure was followed as above described, except that dry pustule contents was 
substituted for the methylene blue. The animal was kept under observation for 
sixteen days. The monkey had a cough from the fifth to the eighth day after the 
inoculation. On the eighth day of the experiment the body temperature rose to 
40°.5 C., and an abundant general exanthem appeared. The lesions of the erup- 
tion passed through an evolution which closely simulated that seen in the lesions 
of a diserete variola vera in man. On the sixteenth day of the experiment the 
animal was vaccinated on the abdomen with virus No. 1. No reaction followed. 
Two other monkeys were inoculated in the lung in the same manner 
and with the same virus. One of these developed symptoms like that 
above described. The third animal showed a distinct rise of temperature 
on the third day of the experiment and was immune to subsequent vac- 
cination but did not develop an exanthem. The same method of inocula- 
tion was followed in another series of 5 monkeys, but powdered variola 
disk was substituted for the mixture of pustule contents and lycopodium. 
Summary.—Two of the animals in the series inoculated with dried 
pustule contents showed an exanthem. ‘The exanthem appeared on the 
eighth day of the disease and was profuse. It one of these animals the 
evolution of the eruption was similar to that seen in variola vera in man. 
A cough was noted in the 2 monkeys which developed an exanthem. 
The constitutional reaction was not marked, but in each animal a distinct 
rise in body temperature was observed. The fever began on the eighth 
day in two and on the sixth day in one. 
The monkeys inoculated by inhalation of pulverized variola disks 
showed no exanthem, no constitutional reaction, and an indefinite tem- 
perature reaction. One animal was found to be refractory to a subse- 
quent skin inoculation with variola virus. 
(e) Attempts to inoculate the monkey by exposure to smallpox 
fomites.—In the series of inoculations previously described we employed 
material the infectiousness of which was demonstrable. In all these 
experiments, although they approximate somewhat the conditions in 
which man contracts smallpox, the amount of contagious material em- 
ployed was excessive. In the series to be described we attempted to place 
the animals under conditions which experience has shown would result 
in an attack of smallpox if man was subjected to the test, although we 
were unable to demonstrate the presence of a contagium. 
No. 243. Adult male, M. cynomologus. This monkey, together with 4 others, 
was kept in a cage in which a blanket was placed which had been wrapped around 
a smallpox patient. The blanket had been in contact with the patient for 6 hours 
