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of degeneration of the organism, and would be unfavorable for the 
production of an immune substance which resulted from the reaction of 
the host cells. 
The high potentiality of the skin as a locus of the initial inoculation 
for immunity production is in sharp contrast with that of the mucous 
membrane, and the physical conditions are in keeping with the interpre- 
tation given above. In a skin lesion the greater part of the products 
of the lesion must be absorbed and go to produce the general immunity. 
It seems probable from the histological study of the specific lesion 
in the nose that this locus would stand nearer to the skin in its poten- 
tiality for immunity production than the lip or palate. We feel that 
our data are insufficient for generalization on this point. 
CONCLUSIONS. 
(1) The degree of protection conferred by a vaccinal or variolous 
lesion on the monkey (M. cynomologus) is conditioned by the locus 
chosen for inoculation as well as by the virus which is employed. 
(2) The varying degree of immunity production which follows the 
development of vaccinal or variolous lesions at different loci of inocula- 
tion is dependent upon the physical conditions there present. 
(3) The outcome of an inoculation of an animal which has had a 
variolous or vaccinious lesion depends upon the locus and upon the 
virus employed in the second inoculation as well as upon the locus and 
upon the virus employed in the first one. 
3. ON THE TIME OF DEVELOPMENT OF THE IMMUNITY AFTER INOCULATION 
OF THE SKIN OF THE MONKEY WITH VACCINE AND WITH VARIOLA 
VIRUS. 
Introduction—The following experiments were planned to show the 
interval which elapses between the inoculation of the monkey’s skin 
with vaccine or variola virus and the development of an immunity 
inhibiting further inoculations. The results of these experiments bear 
upon the general problem of the diseases, and particularly upon that of 
the causation of the exanthem in variola inoculata. The experiments 
are arranged in three series, as follows: 
(a) Daily inoculation of the skin with vaccine virus.—Five monkeys (M. cyno- 
mologus) were selected and each received upon the skin of the abdomen a single 
vaccination daily for 9 days. The development of each lesion was observed and 
objective descriptions recorded from day to day. Vaccine virus No. 148 was 
employed. 
No. 108. The lesions from vaccinations performed on the second, third, fifth, 
sixth, and seventh days of the experiment showed a typical development. Those 
from inoculations on the first and fourth days were abortive in character, vesicula- 
tion not being complete. The inoculations done on the eighth and ninth days 
were entirely negative. 
