4. ars 
306 
THE OCCURRENCE OF CYTORYCTES VARIOLA! IN VACCINIA. 
Cytoplasmic forms are found in every lesion in which there is a 
characteristic process following the inoculation of vaccine. In the vac- 
cination lesions of the skin, cytoryctes are usually demonstrable 48 
hours after inoculation and in a few instances 24 hours after inoculation. 
They persist in the lesions up to 8 days after the vaccination and may 
occasionally be found in small numbers for a longer period. They are 
found at the sides of the vesicles where there is a gradual transition from 
normal epithelium to that in which degeneration is advanced. The 
small, deeply stained, and sharply defined forms are found at the periph- 
ery where the epithelium is nearly normal. The expanded indefinite 
forms which Calkins has described as amoeboid forms, and residual masses 
from which the granules have disappeared are found in older portions 
of the lesions. These indefinite forms are present in lesions of 72 hours 
and in some cases in those of 48 hours duration. In the early lesions 
they are found in relatively small foci, but as the process advances they 
are distributed through a greater mass of the epithelium. ‘They are 
often distinguishable in cells advanced in degeneration, but are not so 
in the fluid of the vesicle. In several lesions cytoryctes were found in 
the endothelium and in other cells about a small superficial blood vessel, 
just beneath the vesicle. 
The process produced by the vaccination of the cornea is accompanied 
in every instance by the cytoplasmic forms of cytoryctes. With the ex- 
tensive destruction of epithelium in these inoculations, a considerable 
surface is denuded so that cytoryctes are present only in small numbers 
at the edge of the degenerating epithelium. 
In the vaccine lesions of the lip, nose, and soft palate cytoryctes are 
constantly present. Their distribution is similar in these lesions to that 
of the skin lesions, the small, sharply defined forms occurring at the 
periphery and the larger expanded forms in the older portion of the 
process. 
In this large number of vaccine lesions produced by the inoculation of 
monkeys with 4 different strains of vaccine virus, the nuclear forms of 
cytoryctes as found in variola do not occur. However, in vaccine 
lesions it is not uncommon to find nuclei. distended with eosin-stained 
material in the form of reticulum, hyaline granules or globules. In 
some cases inclusions of this sort are abundant. The eosin-stained 
masses may have a regular, definite contour, or they may be faint and 
indefinite in outline. The reticular masses usually have an irregular or 
frayed periphery. Greatly distended nuclei may contain a large number 
of rounded globules of uniform size, which in some instances show a 
tendency to vacuolation, or in others may possess deeply staining centers. 
These inclusions often contain masses of deeply stained material, evidently 
the chromatin of the epithelial cells. However, vaccine lesions of the 
skin, the cornea, and the mucous membrane of the lip, nose, and soft 
