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palate, taken at all stages of the process from its beginning up to the 
time of repair, fail to show the presence of the specific nuclear bodies 
found in variola. Two primary vaccine lesions from a native child, 
obtained at 5 and 7 days, respectively, after vaccination, present no 
nuclear forms such as are found in variola lesions. 
THE OCCURRENCE OF CYTORYCTES VARIOLA IN PRIMARY VARIOLA LESIONS. 
Primary lesions produced by the inoculation of Philippine monkeys 
(M. cynomologus) with variola virus are constantly associated with the 
cytoplasmic forms. The distribution of these forms in variola skin 
lesions differs in one respect from their distribution in vaccine lesions of 
the skin. In the process which is present beneath the epithelium in the 
primary variolation, and which involves the dermis and subcutaneous 
tissue, cytoryctes are present in large numbers. They are found with 
endothelial cells in situ within the vessels or lying in the adjacent tissue. 
The endothelium of blood vessels as well as lymphatics is thus affected. 
Three Java monkeys (M. nemenstrinus) afforded skin variola lesions of 
a peculiar type. The process affects but a small area of the epithelium, 
while the dermis and subcutaneous tissue show an extensive lesion. 
Cytoplasmic forms are present in the endothelial cells found in this 
region, also in sections in which they are not demonstrable in the 
epidermis. 
The time occurrence of the cytoplasmic forms is practically identical 
with that in vaccine lesions of the skin. They are present in lesions of 
48 hours, and in some instances in those of 24 hours’ duration. They 
usually disappear from the lesion 9 days after inoculation, but occasion- 
ally they are found subsequent to this in small foci. Their distribution 
in the epithelial portion of the lesion is similar to that in vaccine lesions. 
The nuclear forms of cytoryctes are found in the majority of lesions 
resulting from variolation of the skin of both the Philippine and the 
Java monkey. In no case are these forms numerous. In many the 
study of a single section through the lesion reveals one of several of these 
forms. A prolonged search through a great many sections is necessary 
in other cases to find a single typical nuclear form. They are usually 
found in, cells forming the floor of the vesicle or in those of the hair 
follicles where the degeneration of cells is advanced. Associated with 
the typical nuclear forms are other inclusions which, however, are not 
specific to variola. These appear as masses of stringy reticulum, hyaline 
bodies, granules, and globules within the nuclei, similar to the forms 
already described in vaccinia. They may include portions of the nuclear 
chromatin and may all be duplicated in vaccine lesions, but are not 
nearly so abundant there as in the variola lesion. The reaction of these 
structures to the eosin-methylene blue stain is apparently identical with 
that of the specific nuclear forms. 
In the skin variolations, hyaline masses within the nuclei may show 
a tendency to vacuolation or they may have definite rings embedded in 
