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injection furnishes a greater number of nuclear forms than any other 
experimental lesion, with the exception of the primary lesion of variola 
inoculata in the orang-utan; in this respect it approaches the character 
of the variola vera eruption in man. 
Besides the lesions appearing as an eruption on the surface of the 
body following inoculation with variola virus, similar ones may appear 
on the mucous membranes, or in more remote organs. Following the 
inoculation of the tracheal epithelium of a monkey through an incision 
in the neck, there was not only a profuse eruption upon the skin, but like- 
wise upon the mucous membrane of the mouth, cheek pouches, and cesoph- 
agus, and also in the seminal vesicles. In all these lesions the cytoplasmic 
forms of cytoryctes are present. 
THE OCCURRENCE OF CYTORYCTES VARIOLA IN REINOCULATIONS. 
In many animals which had been inoculated upon the skin with 
variola virus, a subsequent inoculation of the cornea with variola or 
vaccine virus resulted in a typical reaction. The corneal lesions in 
these cases contain cytoryctes which can not be distinguished from those 
of the lesions of first inoculations. 
Lesions produced by the inoculation of dried smallpox or vaccine virus 
appeared later and ran a milder course than was the case with fresh 
virus. In these lesions cytoryctes were present in small numbers, this 
apparently being due to the limited area affected. They were morpho- 
logically identical with those occurring in other lesions. 
SUMMARY AND CONCLUSIONS. 
(1) The cytoplasmic forms of Cytoryctes variole are found constantly 
in all specific lesions resulting from inoculation with variola or with 
vaccine virus. They appear in the primary lesions of both variola 
inoculata and vaccinia soon after the inoculation. They persist in the 
primary skin lesions for about 8 days after inoculation, at which time 
immunity is established and repair is beginning. In variola inoculata 
the exanthem as well as the primary lesions contain cytoplasmic forms. 
(2) Intracellular forms are found within the epithelial nuclei in 
lesions resulting from the inoculation of the monkey with variola virus 
and do not occur in vaccine lesions. These structures are specific for 
variola. Other nonspecific, nuclear inclusions occur in vaccinia, in 
variola, and in other nonrelated processes. 
(3) The nuclear forms of cytoryctes, which are found only in small 
numbers in the primary skin lesion of variola inoculata in the monkeys 
(Mf. cynomologus and M. nemenstrinus), are present in far greater 
numbers in the corresponding lesion of the orang-utan. 
(4) Nuclear forms were only occasionally found in lesions of the 
general eruption following the inoculation of the skin of the monkey 
with variola virus, but were very numerous in the eruption which 
followed the intravenous injection of variola virus into the tail vein, 
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