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inoculated on the skin of the monkey or the cornea of the rabbit. 
Furthermore, the clinical aspect of the disease, now that new cases were 
appearing, made the diagnosis of varicella already indicated by the 
inoculation results, certain. 
Before passing on to the description of the varicella lesions it may be 
well in this connection to give the results of some further experimental 
inoculations made at a later date. The fluid contents of vesicles was col- 
lected from three typical cases of varicella. In two cases the vesicles were 
perfectly clear, in the other one many vesicles were slightly clouded. 
The fluid collected was used to inoculate a series of seven rabbits. ‘The 
mode of inoculation was first to anwsthetize the eye with a few drops of 2 
per cent cocaine solution so that it could be pressed gently from its 
socket and kept immobile while inoculating. In most instances two or 
three shallow parallel incisions were made across each cornea just deep 
enough to penetrate the epithelium. The fluid to be inoculated was 
then placed upon the surface and rubbed gently into the incisions. ‘The 
rabbits were killed at twenty-four hour intervals after from one to six 
days and the corneas fixed in Zenker’s fluid. The seventh animal was 
allowed to live for an indefinite period. 
Rabbit No. 37.—Both corneas were inoculated with cloudy fluid from vesicles 
of case No. 32. The animal was killed twenty-four hours after inoculation; the 
corneal surface was nearly smooth. 
Rabbit No. 38.—kKilled seventy-two hours after inoculation with vesicle from 
case No, 32. The corneal surface appeared smooth and without defect in epi- 
thelium. There was no conjunctivitis. 
Rabbit No. 39.—The corneas were inoculated with clear fluid from vesicles of 
cases Nos. 34 and 35. Killed forty-eight hours after inoculation. The right eye 
showed marked conjunctivitis, and there were several minute whitish spots in the 
surface of the cornea. 
Rabbit No. 40.—The corneas were inoculated with clear lymph from cases Nos. 34 
and 35. Killed four days after inoculation. Corneal surface nearly smooth. 
Rabbit No. 41.—Corneas inoculated with clear lymph from cases Nos. 34 and 35. 
Killed five days after inoculation. Corneas nearly smooth. 
Rabbit No. 42.—Corneas inoculated with clear fluid from cases Nos. 34 and 
35. Killed six days afterwards. Corneal surface smooth. No inflammation. 
Rabbit No. 43.—Corneas inoculated with clear vesicle fluid from cases Nos. 34 
and 35. This animal was not killed. The incisions of the corneal surface 
healed rapidly after the manner of aseptic wounds and no apparent ‘process devel- 
oped during the fortnight in which the animal was kept under observation. 
In the histological study of the corneas of this series of rabbits nothing was 
revealed, with the exception of one case, beyond the usual process of repair which 
follows an uninfected wound. In rabbit No. 39 one cornea showed a marked 
inflammatory process. Microscopically there were collections of bacilli growing 
in the depth of the incision and below the epithelium. The epithelium was thin 
and many cells were degenerated. The adjacent corneal tissue was infiltrated 
with leucocytes. 
In no case did the gross or the histological appearances of the inoculated 
cornea suggest a process beyond that of repair, except one case in which 
there was considerable inflammation. In the latter case the inflammatory 
