536 
intestine there were a few quite small, but rather ragged, superficial ulcers, 
surrounded by dark, pigmented zones. The submucosee was oedematous and 
injected. The mucous membrane of the large bowel as a whole was somewhat 
edematous and injected, that of the small bowel showed no change other than 
a catarrhal one. The mesenteric, intraperitoneal, and internal inguinal glands 
were markedly enlarged, and were, as a rule, pale, but with areas of congestion. 
The great blood vessels showed no abnormality. The pancreas was firm and pale. 
Anatomic diagnosis.—Splenic hypertrophy ; atrophic, hepatic cirrhosis ; 
subacute, parenchymatous nephritis; abdominal, lymphatic, glandular 
hypertrophy ; hypostatic congestion of the lungs; petechiz beneath the 
visceral pericardium ; persistent thymus. 
At autopsy, cultures and smears were made from the spleen, liver, and heart. 
Those from the heart and liver showed no organisms. ‘Those from the spleen 
gave a considerable number of small bacilli, similar to those found in the ante- 
mortem preparation. Cultures on glycerine, agar, and blood serum after forty- 
eight hours showed numerous discrete, minute, translucent, moist colonies. 
Transplants were made on various media with the following results: On alkaline 
and acid agar (1 per cent to phenolphthalein), glycerine, glucose and lactose, agar 
and blood serum, the organism in twenty-four to thirty-six hours gave rise to fine, 
almost transparent, moist columns, which increased but very little in size during 
the four or five days during which they remained alive. In glucose, saccharose, 
and inulin bouillon the growth was scarcely perceptible and took the form of 
a firm, floceulent precipitate, in which the organisms rapidly died out. Milk 
was acidified within twenty-four hours, coagulated in seventy-two hours, the casein 
separating from the whey in six days. On potato, the growth was not visible to 
the eye, but nevertheless it was present. The organism thrived best and lived 
longest in milk and in potato. It did not form indol. This organism, stained 
by Gram’s method, is non-motile and pleomorphic, but when young and vigorous 
it appears as a very small, polar-staining rod: It ferments all of the sugars to 
a slight extent, without gas formation This was indicated by a change in acidity, 
which in the case of glucose required 0.2 cubic centimeter N/20 NaOH, in that 
of lactose 0.1 cubie centimeter, with saccharose 0.2 cubie centimeter, and inulin 
0.25 cubie centimeter per cubie centimeter of the culture media, to neutralize. 
(The titrations were performed with phenolphthalein as an indicator.) 
i} 
A monkey (Macacus cynomologus) was inoculated subcutaneously with 
1 cubic centimeter of a suspension of the organism from an agar culture 
seventy-two hours old. A second monkey was inoculated in the same 
manner with 1 cubic centimeter of a suspension from a seventy-two-hour 
blood serum culture. The results, as far as the temperature was con- 
cerned, are shown by Charts Nos. 2 and 5. The organism used in the 
case of monkey No. 4 had been grown in blood serum for six generations. 
The constitutional symptoms were most marked in No. 1, which lost its 
appetite completely after a few days. This monkey was killed on the 
seventh day after inoculation. The spleen was not enlarged and the 
other organs showed no microscopic changes. Cultures from the blood 
and spleen were made upon agar, bouillon (—1 to phenolphthalein), 
and blood serum. No growth occurred in those from the spleen, but in 
the ones from the blood upon agar and blood serum a slight one occurred 
