_ 280 The Philippine Journal of Science 1922 
On microscopic examination Reinking found the organisms to 
be abundant in leaf veins and exuding from those elements 
in mounted sections. No studies were made with pure cultures 
of the organism. 
The above description of symptoms is very similar to that of 
Burkholder. (1) 
Reinking(5) reported the presence of angular leaf spot of 
cotton caused by Bacterium malvacearum E. F.Sm. He says of 
this disease: 
The disease is present on leaf, stem, and fruit. On the leaf the char- 
acteristic spots are from 1 to 4 millimeters in diameter; they are angular 
with brownish centers bordered with light brown to yellow. Young spots 
are similar and have a water-soaked appearance. * * * The disease 
mby be evident on the tender stalks in the form of blackened cankerous 
patches. On the bolls, at first, minute water-soaked spots are produced, 
which later may run together, producing sunken brownish or reddish 
brown blotches. 
Furthermore, Reinking states that “The causal organism is a 
bacterium that produces a yellow pigment in pure culture.” No 
proof is given to show that the organism forming the yellow 
pigment is the pathogenic or the saprophytic one commonly 
associated with the disease. 
The descriptions of the symptoms of the disease compare 
favorably with those of Rolfs,(6) McCall,(4) and Faulwetter. (2) 
While the descriptions of symptoms are valuable they do not 
furnish proof of the identity of the causal organism. Because 
of the accuracy of the preceding descriptions they will suffice | 
for the present paper. The physiological and morphological 
studies carried on with the various organisms are here presented 
briefly. In each case inoculations under controlled conditions 
were carried out to prove the pathogenicity of the organisms. 
BACTERIUM (BACILLUS) SOLANACEARUM E, F. Smith. 
The organism was isolated from wilted tobacco, eggplant, and 
tomato plants. Isolations were made by crushing the material, 
after treatment for one minute in corrosive sublimate, 1 to 
1,000, in sterile water, and plating directly in nutrient agar. 
_ The organism stains readily with the common aniline dyes 
and shows no irregularity in taking the stain. The cells 
measure from 0.8 to 1.2 » in length. 
According to Smith(7,10) the cells measure 0.6 to 1.0 » in 
length with several peritrichous flagella. 
The artificial media used were titrated to + 10 Fuller’s scale. 
