20, 8 Welles: Bacteria Pathogenic to Plants 281 
Nutrient agar slant.—After twenty-four hours, growth was 
abundant, filiform, convex, dull, smooth, opaque, dirty white, 
without odor, and slimy. After ten days the growth became 
brownish, with a slight brownish coloring of the agar. Accord- 
ing to Smith (7, 10) the growth was white, smooth, moist, glisten- 
ing, becoming yellowish brown to brown and the agar was 
stained brown. 
Nutrient agar colonies—After twenty-four hours, rapid 
growth had taken place at ‘26° to 28° C., with colonies round, 
smooth, convex, entire-edged, and finely granular internal struc- 
ture. 
Potato slant.—After twenty-four hours, growth was moderate, 
filiform, slightly convex, dull, smooth, brownish, no odor, and 
slimy. After a week, the growth became a deep brown. Ac- 
cording to Smith(7,10) the growth was dirty white becoming 
brownish to smoke-black. 
Sugar media.—Neither gas nor acid was formed in saccharose, 
dextrose, lactose, or mannite. Smith(10) reports that acid and 
gas are not produced in common sugar media. 
Nutrient broth.—After twenty-four hours, growth was abun- 
dant, surface growth was more or less flocculent, becoming evenly 
distributed throughout the medium on agitation. Clouding was 
moderate, without odor. A slight sediment was formed and an 
alkaline reaction was obtained. According to Smith(7,10) zo- 
ogloea developed in the upper layer, giving the medium uniform 
turbidity on agitation. An alkaline reaction developed. 
PSEUDOMONAS PHASEOLI E. F. Smith. 
The organism was isolated from diseased bean leaves (Pha- 
seolus vulgaris Linn.) by crushing the material in sterile water 
and plating directly in potato agar. 
The bacterial cells stain readily with the common aniline dyes 
and appear as short rods with rounded ends. Smith(8) states 
that “Bacillus phaseoli E. F. S. is a short rod with rounded 
ends, * * * motile in early stages of growth.” 
All artificial media were titrated to + 10 Fuller’s scale. 
Nutrient agar slant—After twenty-four hours, growth was 
moderate, entire-edged, convex, glistening, smooth, opaque, yel- 
low (chrome), with an odor of slight putrefaction, and of slimy 
consistency. 
Potato agar colonies —After three days, growth was moderate 
and colonies were round, smooth, convex, edge entire, internal 
