282 The Philippine Journal of Science 1922 
structure finely granular. The colonies were yellow with hyaline 
center. The starch was reduced by the action of the bacteria. 
Smith(9) states that copious and prolonged growth occurred, 
covering the potato plug and developing in the water, and within 
a few weeks the starch was hydrolized. 
Nutrient broth—After twenty-four hours, there was a slight, 
tough surface growth, which sank on agitation. There was 
slight, even cloudiness throughout the medium. 
Lactose broth.—There was slight flocculent precipitate with no 
acid and no gas. 
Dextrose broth.—There was considerable surface growth. No 
gas was produced; the reaction was strongly acid. 
Saccharose broth.—There was a moderate surface film not 
breaking up on agitation. No gas was produced. 
Mannite broth.—Flocculent precipitate developed in the me- 
dium. There was no gas, and the reaction was slightly acid. 
Smith(9) states that on agars containing various sugars growth 
was copious. 
Glycerine broth_—There was a flocculent growth and the me- 
dium was heavily clouded. No gas was produced, and the re- 
action was slightly acid. : 
Nitrate broth—There was a moderate flocculent growth. 
Neither gas nor acid was produced, and there was no reduction 
in four weeks. 
Nothing has been published, to my knowledge, giving the com- 
plete growth characters of this organism. There have been a 
few reports where partial culture work has been given. As the 
descriptive chart of the Society of American Bacteriologists is 
being followed in this paper to a large extent, the cultural char- 
acters in Smith’s famous paper of 1901 permit of very little 
comparison. However, from the symptoms and the checking ofa 
few comparative factors the organisms as well as the diseases 
seem identical. 
BACTERIUM MALVACEARUM E. F. Smith. 
The organism was isolated from young, watery lesions on 
leaves of cotton, by the method given above. The cells stain 
readily with the common aniline dyes, showing no irregularity 
in taking the stain. 
_ Nutrient agar colonies—After twenty-four hours, growth 
was moderate (26° to 28° C.). The colonies were round, smooth, 
convex, entire-edged, with internal structure finely granular, with 
