20, 8 Welles: Bacteria Pathogenic to Plants 283 
a diameter of 1 to 3 ». A pale yellow pigment was produced. . 
According to Rolfs,(6) the amount of growth appeared on dif- 
ferent sugar media in the order named; dextrin, mannite, mal- 
tose, and dextrose, and a pale yellow pigment was produced on 
agars. 
Nutrient agar slant.—After twenty-four hours, growth was 
moderate, filiform, raised, glistening, smooth, opaque, pale yellow, 
odor of putrefaction, and a slightly slimy consistency. 
Nutrient broth.—There was a thin surface growth. Culture 
was moderate and uniformly clouded and no sediment was 
formed. 
Lactose broth.—A light surface growth appeared. 
Dextrose broth.—There was moderate surface growth, with no 
gas, and a strongly acid reaction. 
Saccharose broth—There was a surface growth and a mod- 
erate, uniform clouding of the liquid. No gas was formed. 
Mannite broth—No gas was formed, and the reaction was 
strongly acid. 
Glycerine broth—There was no gas and no acid. Clouding 
was slight. Rolfs(6) states that growth was poor on glycerine 
agar. 
The description of the organism by Rolfs checks with the or- 
ganism just described, excepting that no difference in degree of 
growth was observed on the various sugar media. The nonpath- 
ogenic organism producing the bright yellow pigment, men- 
tioned by Rolfs, was frequently encountered in isolations, and 
it caused considerable trouble as it appeared before the patho- 
genic pale yellow organism. 
BACTERIAL WILT OF PARSLEY 
The bacterial wilt of parsley, which has been found to be 
caused by a new species of bacteria, was for several seasons 
believed to be caused by Bacillus solanacearum E. F. Sm. The 
vascular bundles, on microscopic examination, appeared to be 
packed with bacteria, and the whole behavior of the parasitized 
plants was precisely like that of solanaceous ‘plants parasitized 
by Bacillus solanacearum E. F. Sm. A further substantiation 
of this diagnosis was that the diseased plants were found on soil 
which was known to be heavily infested with the solanaceous 
wilt organism. On making physiological studies, the organism 
proved to be entirely different from Bacterium solanacearum 
