284 The Philippine Journal of Science 1922 
, BACILLUS NELLIAE sp. nov. 
The organisms are short rods with rounded ends, 0.83 to 2.27 pe 
by 0.37 to 0.50 ». They stain readily with all common aniline 
dyes, and show no irregularities in taking the stain. The ther- 
mal death point lies between 53° and 54°. Three to seven peri- 
trichous flagella have been demonstrated. The artificial media 
were titrated to + 10 Fuller’s scale. 
Nutrient agar stroke——After twenty-four hours, growth was _ 
moderate, filiform, flat, more or less glistening, contoured, 
whitish by transmitted and translucent by direct light, without 
odor, and of a slimy consistency. With age the culture became 
irregularly raised. 
Nutrient agar colonies—Surface colonies after twenty-four 
hours were round, slightly concentrically ringed, raised, finely 
granular in structure, and with an entire edge. 
Potato agar slant.—Growth was moderate, spreading, with 
yellow pigment, flat, smooth, odorless, and of viscid consistency. 
Nutrient agar stab.—There was moderate arborescent growth, 
and considerable surface growth. 
Potato-glucose agar stroke.—After twenty-four hours, growth 
was abundant, filiform, slightly convex, glistening, translucent, 
becoming opaque, and of a viscid consistency. Gas was formed 
under the medium forcing the latter up in the tube. 
Dextrose broth.—Acid and gas were produced. 
Lactose broth.—Gas was produced. The reaction was slightly 
acid. 
Galactose broth—Gas was formed. A strong acid reaction 
was obtained. 
Saccharose broth—Gas was formed, and the reaction was 
strongly acid. 
Mannite broth—Gas and a strongly acid reaction resulted. 
Glycerine broth—Neither gas nor acid was formed. Growth 
took place in the closed and open arms of fermentation tubes. 
Nitrate broth.—There was no reduction; neither gas nor acid 
was produced. 
Toleration of sodiwm chloride—Growth occurred at 8 per cent 
concentration. 
Effect of sunlight.—Twenty minutes’ exposure reduced but 
did not inhibit growth. 
LITERATURE CITED 
1, BURKHOLDER, WALTER H. Bean diseases in New York State in 1916. 
Abs. Phytopath. 7 (1917) 61. 
