20, 3 Lee: Citrus Tissues and Citrus Canker 335 
TABLE 3.—Kesults of inoculation with Pseudomonas citri on Washington 
navel orange fruits of various degrees of maturity—Continued. 
Fruit. : Infections, 
Needle 
4 punc- 
No. Age.@ — tures. era Not at punctures. 
Days. mm, Per cent. Number, 
a Re che on ES EE 86 57.0 20 90/4 
es 2s oe 86 50.0 20 90/6 
| DERI « Saaae exe SBeeaeY te ie 86 48.0 20 95 | None. 
BES oooh ca ane shan cuee tees ee 101 60.0 20 100 Do, 
BE a Se eo se ee 101 55.0 20 100 | 4 
BM Boe sock oasis eee Gee 101 50.0 20 100 | 2 
$655. 355s ee 5 ae oe es 101 57.0 20 100 | None, 
|: geet Ae aR AEP Hoes 101 52.0 20 100 Do. 
FG oe ou eee eas tees 101 63.0 pp peceuemes toa Do. 
sy ae Gear male, coger EEE: ot 101 66.0 | Peer Do. 
«5 Seana ts SRBC Sok OK eee oan eae oe 101 54.0 Be aati bi 05 oe Do. 
WW ncpnscceneces antes abt eeecede 101 54.0 DP fevkoweesan Do. 
We as nies ad pean a ees adGS 101 50.0 ie Pataca neler De. 
BE paws Gea oe eee cae 117 46.0 20 0 Do. 
se cc toe cs cae 117 50.0 20 (>) Do. 
REP Salen ots Mente ores gear Th 117 50.0 20 e25 Do. 
6 ee a ee 117 48.0 20 ©25 Do. 
Mibinisic sta cegede ee sac nik 117 50.0 20 ©40 Do. 
Oe a ea ee 117 52.0 20 ©40 Do. 
»y Giga Ne tetepi eer Berea so MeeVee <r! 117 48.0 20 ©25 Do. 
OS oe a ee 117 50.0 20 ©60 Do. 
Beer Re SCRE ete Rar eare ae Ee 117 48.0 20 ©60 Do. 
| | peel ane a Cen eere rear as gee 117 48.0 20 ©60 Do. 
91 AOR Tre ee ay tes 130 69.0 20 0 Do. 
We 130 66.0 20 0 Do. 
<P aero eer See ORE on mee red 130 66.0 20 0 Do. 
gt Ne ee ee 130 69.0 20 0 Do. 
RISER Tt OG RES. 130 69.0 20 c4 Do. 
a ae ea eer 130 76.0 0 0 Do. 
| f Skt RIE Oe Re eT Semi e ate He 130 63.0 0 0 Do, 
Oe a i 130 69.0 0 0 Do. 
_ | Bante carole Diataliie eueain annon s 130 63.0 0 0 Do. 
0 oe Sues 130 66.0 0 0 Do, 
“It is difficult to determine practically the period at which the ovary of the flower becomes 
a fruit; in this case, fruits 1 to 20 were inoculated but one or two days after the petals had 
dropped. 
> Fruit lost. 
© Although these punctures are recorded as yielding positive results, nevertheless the 
only reaction obtained was a yellow discoloration without actual canker formation. From 
an orange grower’s viewpoint no canker resulted. 
age as well as to approximate their stages of maturity by their 
sizes. Throughout the season similar technic was used for the 
inoculation, and the inoculum was always obtained from 7- to 
10-day potato cylinder cultures. All inoculations were main- 
tained under identical environmental conditions. The results 
are recorded in Table 3. 
