A478 The Philippine Journal of Science 1922 
is smooth; while the cells of V. globator are angular in surface 
view and connected by relatively stout protoplasmic processes, 
and the outer wall of the oospore is verrucose with conical 
warts (West, 710). A more fundamental difference between 
these species was demonstrated by Meyer (’96) in his investi- 
gation of the structure of the membranes of these organisms; 
a difference so great, in my estimation, as to warrant the sepa- 
ration of V. aureus from Volvoxz, which I now propose, under 
the name Janetosphaera genus novum, based on Meyer’s text 
figs. 3 and 4 (reproduced herewith as text figs. 1 and 2) of 
the cell membranes as compared with his text figs. 1 and 2 
(reproduced herewith as text figs. 3 and 4) of the corresponding 
structures of V. globator. For a clear recognition of the true 
status of J. aurea (Ehrenberg) Shaw it is desirable that 
membrane studies as detailed as those of Meyer be made to 
cover all phases of the life histories of these and other species 
of the larger Volvocaceae. Some specimens collected at Stanford 
_ University, California, in 1896, and still in my possession, will 
be described in this paper. They present the characters given 
by Klein (’89A) and by Overton (’89) for this species, and 
testify to its occurrence in western North America. 
Of the newer species, V. perglobator (Powers ’08) has vege- 
tative cells very similar to those of V. globator, from which it 
is distinguishable by having a larger number of oospores, which 
often exceed 100 as compared with a range of from 12 to 40, 
and by having the outer wall of the oospore crenate. Volvox 
rousseleti (West ’10 and ’18) is readily separable from V. glo- 
bator by the greater number of cells, 25,000 to 50,000; by the 
constant number of gonidia, 8; by the larger number of oospores, 
120 to 150; and by the dense covering of spines on the spores. 
For V. perglobator no estimate of the number of cells is given by 
Powers, so we are left to assume that its range of variation in 
this respect is similar to that of V. globator. 
In the vicinity of Manila, during the latter months of the 
rainy seasons of 1914 and 1915, I made collections which in- 
cluded several species of the higher Volvocaceae. Some of these 
have vegetative cells without protoplasmic connections, and 
display developmental characters which make it very question- 
able whether any of these organisms lacking the protoplasmic 
connecting strands between their cells should be regarded as 
species of the genus Volvoz. They have been or are being de- 
scribed in other papers. Others, the subjects of this paper, do 
have protoplasmic connections between their cells. 
