20, 5 Shaw: Janetosphaera and Volvox 493 
brane (toward the center of the coenobium) of the somatic 
cells is visible in the specimen. The connecting filaments 
mostly show a pair of nodes near their middles. There is but 
one direct filament between any two neighboring protoplasts, 
but some protoplasts send out one filament to a neighbor and 
another to a filament connecting that neighbor with a third 
protoplast, and others send out two filaments which unite with 
two processes connecting the same neighbor with two other pro- 
toplasts. ; 
The oospores in this specimen were all sketched in outline 
with a camera lucida and counted. The number is 92. There 
are none in the anterior fourth or fifth of the colony. They 
all lie near the periphery of the colony, 29 to 39 » below the 
outer membrane. They are fairly evenly distributed in the 
area which they occupy, though showing considerable grouping 
in pairs, and leaving vacancies at what I suppose to be the sites 
of antheridia, of which there are about three on the upper and 
the same number on the lower side. 
The outer wall of the spore is developed into hollow spines, 
of which peripheral counts in the median optical section give 
from 14 to 16, averaging 15, a number which corresponds to 
a total of about 82 on the spherical surface. These spines are 
nearly 11 » high, and their bases are close together. The basal 
two-fifths of each spine is broadly conical, most of the re- 
mainder is more narrowly conical with a tip which is more 
broadly conical. Many of the spines are somewhat inclined to 
one side. The thickness of the wall of the spines does not 
Show clearly with the highest power that can safely be ap- 
plied, but this wall does not seem to be thick. It has a brownish 
yellow color. The diameters of the spores measured between 
tips of spines are about 60 », between bases of spines about 40 xy. 
The oospores measure in diameter 30 to 32 », averaging 31 p. 
The protoplasm’ is densely granular and yellowish. Within 
it there is a dark red spherical body which seems to be the 
nucleus. It may have been stained with some stain the use of 
which was not recorded. Its diameter is a little more than 
two-fifths that of the protoplasts, and it is located excentrically 
about one-fifth of the protoplast diameter from one side. Close 
beside this red body there is a clear space or transparent body 
about half as thick as long, its length being a little less than 
the diameter of the red body. It lies somewhat nearer to the 
periphery of the protoplasts than does the red body. 
185588———2 
