494 The Philippine Journal of Science 1922 
More or less directly over each oospore, seen in surface view 
of the colony, there is a vacancy in the layer of somatic cells, 
the site of the cell before it became the egg or egg apparatus. 
There are other, larger vacancies, as before mentioned, of which 
two show plainly in the photograph (Plate 1, fig. 1), that are 
Supposed to mark the sites of antheridia. A few cells here 
and there in the somatic layer are larger, having diameters two 
or three times as great as the average for somatic cells. 
The specimen is in good condition, the greatest damage it has 
suffered being a crease or furrow formed on the right side, from 
the equator forward halfway to the anterior pole, by a grain 
of sand which lies in the deeper end of the furrow. 
The material from which this specimen was taken was col- 
lected from a seasonal pool (carabao wallow) about 5 meters in 
diameter and 80 centimeters deep, without inlet or outlet, in a 
grassy field in Pasay, about 1 kilometer south of Manila, Sep- 
tember 16,1914. At the time the pool was about half emptied by 
evaporation and seepage. The pool was designated by the 
letter A for the purpose of labeling the material taken from it 
on this and later dates.* 
In several mature female coenobia on the same slide with the 
type specimen there is occasionally a smaller oospore among the 
others. One such is shown in Plate 2, fig. 5. This is from a 
coenobium containing 77 oospores. ‘The large oospores measure 
about 42 », exclusive of the spines, and the spines are about 
11 » high. The spores measure about 64 p Over all. The small 
spore measures about 37 », without the spines, the spines are 
about 6.5 » high, and the width of the spores over all is about 
50 ». The wall and the spines of this small spore are thinner 
and the spines less crowded than on the larger spores. The walls 
of these small Spores resemble those of the next species to be 
described. 
The numbers of oospores counted in some of the coenobia on 
the same slide with the type specimen were: 129, 92, 90, 77, 67, 
60, 57, 47, and 38, the larger numbers being in larger coenobia 
and the smaller numbers in smaller ones. 
: * The slides bearing the type specimens of this and of the other Philip- 
pine species of Volvor are in my possession. Slide mounts of material 
from the same collections have been sent to Prof. Frank G. Haughwout, 
Bureau of Science, Manila, and to Prof. Douglas H. Campbell, Stanford 
University, California. Material from the type locality, bottled in glyc- 
erine, has been sent to sixteen biologists in North America and to sixteen 
in Europe and Asia. Duplicates of this bottled material are available for 
distribution from my American address; Claremont, California.—W. R. S. 
