498 The Philippine Journal of Science 1922 
spines, is about 43 »; excluding the spines, about 34 ». The 
. spines are about 3.5 to 5.5 » high. 
The oospheres vary, probably due to difference in degree of ma- 
turity, some nearly filling the spherical lumen of the spore wall, 
others being excentrically shrunken-away from the wall, the lat- 
ter being commoner in the posterior part of the colony. The 
protoplasm is coarsely granular in most of the oospores, and 
stained reddish, and the nucleus cannot be distinguished. 
More or less directly over each oospore, seen in surface view 
of the colony, there is a vacancy in the layer of somatic cells. 
There are a few other vacancies, one on the upper and two on 
the lower side, in the layer of somatic cells, that are supposed 
to represent antheridial sites. Enlarged somatic cells are not 
noticeably present. , 
The specimen has a break running across the near side a 
little forward from the oosporic region, and a \-shaped crease 
in the middle of the near side. Fungus hyphae on the left 
side of the anterior pole are a reminder of the futility of de- 
pending on exposure of glycerine to the atmosphere in shallow 
dishes for the purpose of evaporation. Some of these hyphae 
penetrate the colony. There are others on and in the lower 
side. 
The material from which this specimen was taken was collected 
from a flood pool near the Pasig railway station, about 10 kilo- 
meters east of Manila, at some time between July 19 and August 
31,1914. The collection was designated by the Roman number 
V for the purpose of labeling slides prepared from the collection. 
Scattered over the surface of the colony, embedded in the 
membranes, are numerous specimens of an endophytic alga 
that is considered to belong in the genus Chlorosphaera Klebs. 
A great variety of stages of the life history of this organism 
are present. The most conspicuous are the resting spores, of 
which a dozen are to be seen in Plate a; es ae 
Six other equally mature female coenobia of about the same 
size and with about the same number of spores are present on 
the same slide with the type specimen. There are also two 
smaller ones with mature oospores, one about 545 by 708 » 
with 73 oospores and the other about 430 by 516 » with 65 
oospores, 
Immature female coenobia are also present. One measuring 
580 by 600 » has the oogonia about 20 » wide, and another about 
885 by 915 » has oogonia about 25 » wide. In the former the 
oogonia are only partly somewhat pear-shaped, in the latter 
a NPN ss ae as celal a gs 
