20, 5 Shaw: Janetosphaera and Volvoz 499 
they are mostly so. In the former five or six partly developed 
antheridia are visible, and in the latter four can be seen, three 
of them sperm globoids and the other cup-shaped and probably 
immature. The preparation is not suitable for observing the 
details of the antheridia. 
A nearly mature asexual coenobium with eight symmetrically 
arranged daughters is shown in Plate 5, fig. 15. This is on 
the same slide with the type specimen and therefore from the 
Same collection. It is compressed under the cover to about 
450 » and measures about 950 »w in diameter. The number of 
cells in the mother was estimated variously at 30,000 and 38,000, 
and those in one of the daughters were estimated at about 26,000. 
This daughter is about 300 » in diameter and has ciliated cells 
about 3.5 » in diameter and closely crowded. It has also a 
- few reproductive cells of about 10 w. The layer of the somatic 
cells of the mother and their membranes is about 23 p thick 
and the protoplasts are in the outer half of this thickness. The 
protoplasts in the front of the coenobium are larger, ovoid, 
and about 3.5 » in diameter. Those at the back are smaller, 
pear-shaped, with the outer half about 1.2 and the inner half 
about 2.5 » wide. In length they are as large as the forward 
' Somatic protoplasts. The protoplasts are spaced about 7 p at 
the back and about 10.5 pat the front. The connecting filaments 
are delicate. 
On the same slide there are other mature asexual coenobia. 
One shows a rear view of a beautifully symmetrical group of 
eight daughters, two others show oblique views of the same 
number of daughters. Two others show seven large daughters 
each, but with a vacant space among the daughters. One mother 
with half-grown and one with one-fifth-grown daughters have 
also eight each. So eight seems to be the number of gonidia 
characteristic of this lot of material. 
From a pond, C, in Pasay, several kilometers distant from 
the source of the type material, there was collected on September 
20, 1914, and fixed on the next day, material of Volvox barberi 
that included asexual coenobia having the number of daughters 
variable, 
One, shown in Plate 5, fig. 16, has only four daughters. They 
are distributed around the equator of the coenobium. The 
Specimen presents nearly a side view and measures in the pho- 
tograph 540 by 580 ». The approximate correctness of the 
Magnification of this figure is indicated by the scale reproduced 
below the figure. This scale is a reproduction of a stage mi- 
