600 The Philippine Journal of Science 1922 
PREPARATIONS EMPLOYED 
We employed alcoholic and aqueous preparations. The for- 
mer was kindly given to us by Dr. P. Valenzuela, of the School 
of Pharmacy, University of the Philippines. It was prepared 
by him from the pulverized mature seeds of the plant, in accord- 
ance with the processes prescribed in the ninth decennial re- 
vision of the United States Pharmacopeia for the preparation 
of the fluidextract of belladonna, except that the last percolate 
was evaporated at 35° to 40° C. under low pressure. The 
latter was prepared as follows: Twenty mils of the alcoholic 
preparation were made alkaline by the addition of about 1 mil 
of 10 per cent ammonia, and then 30 mils of chloroform were 
added, and the mixture was shaken continuously for ten mirutes. 
The mixture was allowed to stand in a separatory funnel, and 
the chloroform layer at the bottom, amounting to 32 mils, was 
removed. This was acidified by the addition of about 2 mils 
of dilute sulphuric acid and then 16 mils of water were added. 
This mixture was shaken continuously for ten minutes, allowed 
to stand in a separatory funnel, and the upper layer was taken 
off. The aqueous layer, after it was made neutral to litmus 
paper by the addition, drop by drop, of 10 per cent ammonia, 
amounted to approximately 21 mils. This aqueous solution was 
then allowed to evaporate in front of a rapidly revolving elec- 
' tric fan to 20 mils. The residue was slightly turbid and yellow- 
ish in color. The concentration of this aqueous preparation 
would be equal to the fluidextract if the active principles were 
completely extracted. We used this preparation in most of 
our experiments. 
SYMPTOMS PRODUCED IN DOGS AND CATS 
Hypodermic injection of 2 mils of the aqueous preparation 
per kilogram of weight of dog or cat chiefly affects the cere- 
brum, the respiration, the pupils, the pulse rate, and the se- 
cretion of tear and saliva. Within fifteen minutes after ad- 
ministration, the animal usually becomes restless and irritable 
when touched; the pupils dilate; the salivary secretion ceases, 
the respiration and the pulse are accelerated. The restlessness 
is followed by incodrdination, then by sleep, and eventually by 
narcosis. The rate of the respiration is slowed as hypnosis sets 
in, but the mydriasis, dryness of the mouth and the conjunctiva, 
and rapid pulse persist even if the hypnosis lapses into nar- 
cosis. One of our protocols showing these different effects of 
Datura alba is given as Table 1. 
