20, 6 Mendiola: Weight and Ash Content of Tobacco 647 
TABLE 11.—Total dry weights and percentages of total ash. 
Humid. Dry. 
Plant No. 
Dry Dr. 
matter. | Ash. watter: Ash 
g P. ct. 9. P. ct. 
parce igighcs Wiatd i edt epee ME nk Pe Rah Sila 3.3179 4.64 2. 4083 5.31 
i eee ae nat SORRY LP Ce OTHE de ECPI GO OO A 3.3480 5.41 2.5902 5.74 
Dea A ee cea een ae cee ee ee 2.7304 4.75 8. 5023 5.07 
Biiremuny cita Speesceaiccatobe slactetaasaccangutenTe oocetsie 1. 4705 7.52 2. 5600 5.16 
ae apg Slee a OEY MSF PREP: Gey reece pe 8 oma PTET PFN 8.1101 5.08 3.1557 5. 65 
Wars i a se a Sg ee ae gee 2. 4512 6.36 3.4154 5.54 
AVOEDG. cscs a eae ee 2. 7380 5. 63 2. 9361 5.39 
i 
| Probable ervor 0f Mean ss oo es ae +0. 1790 £0.28 | 40.1206 | +0.06 | 
CROP II 
Crop II consisted of two series; one series was kept in the 
same chambers in which Crop I was grown and is a repetition 
of Harvest I of that crop. This repetition was thought to be 
necessary as in Harvest I of Crop I no ash determinations were 
made of individual plants, and so the individual variation as 
well as the probable error of the average could not be determined. 
The second series of Crop II was grown in glass chambers also 
in which temperature and relative humidity were not supposed 
to be the factors controlling the relative rates of transpiration 
but rather the different intensity of illumination. One of the 
chambers was covered with white canvas cloth, and the other 
was left uncovered. The doors of both rooms were left open a 
little throughout the experiment to allow some circulation of 
air. The opening, however, did not interfere with the method 
of shading. Each chamber had a capacity of about 500 cubic 
decimeters. 
The seeds used were from the same lot of seeds as those used 
in the first crop. This time, they were germinated in previously 
well-mixed sand in a seed box. The culture solution was pre- 
pared according to the formula that was used in Crop I, but the 
culture flasks were 250-cubic-centimeter Erlenmeyer. Change 
of solution and replacement of water lost by transpiration and 
otherwise were more frequent. 
SERIES I. UNDER HUMID AND DRY ATMOSPHERE 
Seeds germinated, July 23, 1917. 
Seedlings transferred to culture flasks, July 30, 1917. 
Plants placed in culture chambers, August 20, 1917. 
' 185775——4 
