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eubie centimeters of virulent blood were injected, the result being that a typical 
reaction was observed on the second day, followed by death two days later. At 
autopsy, typical, but advanced, lesions were observed. This result seemed to 
indicate that the disease was caused by the filtrate, so that, had the larger dose 
of blood not been used, the animal might have recovered. The fourth bull suffered 
a typical attack of rinderpest. 
The conclusion must be drawn from these experiments that small 
doses of blood or filtrate were not satisfactory with the cattle we used 
because, as was indicated above, their natural resistance is a great one. 
For this reason the quantities were increased in later experiments. 
In the next series fresh, citrated virulent blood was diluted with five times 
its own volume of a 0.4 per cent saline solution and then filtered through a 
new, sterilized Pasteur-Chamberland bougie, under a pressure of 3 kilograms 
per square centimeter. Two native (Sibuyan) cattle received 30 cubie centi- 
meters each of the filtrate and a control animal was given, subcutaneously, 
5 cubic centimeters of the undiluted blood. On the fourth day after inoculation, 
the control (Chart 5, No. 931) developed the disease; it was subsequently bled 
to death and the diagnosis established. One of the others (Chart 6, No. 938) 
had a rise in temperature on the seventh day, was bled to death three days later 
and the diagnosis of rinderpest confirmed; the second one (Chart 7, No. 937) 
reacted on the eleventh or twelfth day and developed diarrhea on the fourth day 
thereafter, whereupon it was bled and the diagnosis of rinderpest made. 
These cases, in view of our other experiences with small amounts of 
virulent blood and delayed reaction, seem to suggest the virulence of 
the filtrate. It should be added that the animals were kept under 
similar conditions in regard to their food and environment but that 
the control was separated from the other two animals by a distance of ap- 
proximately two miles and that no one in attendance upon the one had 
any access to the others. In addition, ten other non-immune cattle which 
did not contract the disease were stabled with the animals receiving the 
filtrate, so that their quarters had not become infected. 
A third series was as follows: An animal received 30 cubic centimeters of 
filtrate from a Chamberland I candle (3 kilograms pressure per square cen- 
timeter). A reaction was observed on the tenth day but on the third one there- 
after the temperature again was normal. After receiving 5 cubic centimeters of 
virulent blood, this bull showed a typical reaction, developed rinderpest, and died. 
Apparently, to judge from these results, the causative agent of the 
disease may or may not pass through the pores of the Pasteur-Chamber- 
land filter according to the conditions surrounding the experiment. 
More work on this subject is therefore necessary. 
Eaperiments with defibrinated blood and with filtered bile-—Various experi- 
ments have been made with defibrinated blood. Nicolle and Bey have published 
the statement that filtered, defibrinated blood will not kill and will not give rise 
to any immunity. Kolle, Turner, and Maberly agree with this view and add that 
the residue on the filter may be virulent. 
