After fifty-six days: 
Stem Kokubo, Abundant growth. Very advanced liquefaction. 
Herzog (large), scanty growth, no liquefaction, 
Herzog I, very advanced liquefaction, like stem Kokubo. 
Okata I, 11, IIT, no liquefaction. 
Control tube, no liquefaction. 
The original communication states that the kakke coccus grows in milk 
without coagulating the casein. Our tests in this respect show: 
After twenty-two days’ growth in litmus milk: 
Stem Kokubo, media fluid, red, little coagulation. 
Herzog (large), fluid, red, moderate coagulation. 
Herzog I, fluid, red, marked coagulation. 
Okata I, II, II, fluid, red, very little coagulation. 
After 56 days, all litmus milk tubes were red and the degree of coagulation 
was as follows: 
Kokubo, moderate coagulation. 
Herzog (large), marked coagulation. 
Herzog I, marked coagulation. 
Okata I, I, 111, marked coagulation. 
Therefore, after a longer period of growth, all stems showed marked 
acid formation and a varying degree of casein coagulation. The control 
tube after fifty-six days had shown no changes. On the other media 
the cultures presented the features already described by Kokubo and 
Okata. None of them formed any gas in glucose or lactose media. 
The following animal experiments were made: 
First series—On November 20, 1905, twelve monkeys (species Maca- 
cus cynomolgus) were inoculated with the 6 stems, as follows: 
Animal number, | Date. | Amount. Culture. . | Stem. 
Monkey No.— | | e¢. | 
1 Yj: Se Re | Noy. 20,1905 1 5-day-old bouillon culture, | Kokubo. 
| second generation of 
original Japanese eul- 
| ture, intraperitoneally. 
KY: Ae ee eme Bene dO Stee Li 2 feats Ge sme ec Do. | 
2 (eee eee tear ee (6 (oem > ares ot Tiree ESV 4 a Herzog (large). 
KE gf Pins ea eal eR (a oye Baga be ce EC) NEG. ee, 5 a rr Do. 
a UN Ae oe Se ae | Pa cof Jane ueaat | Lo a (oy hee Sane, ik aa ea Herzog I. 
1! rc CE RENE Se Pare Ogsestes | j ee Eee pO” a re | Do. 
PARTE ee oe ty Nov. 22,1905 | 1 2-day-old bouillon culture, | Okata I. 
second generation of 
| original Japanese cul- 
ture, intraperitoneally. 
X.Y Ne ee a(R dG. t belie, Mite [ise at Ci (1) ASO ee a er Do. 
| cS See a | nae dae 1s 24 ioe (liga 2e2 Si ed Okata II. 
ut) b eee eee ee eee: CO meas | 1 6-day-old bouillon culture, | Herzog I. 
| second generation of ori- 
+ ginal Japanese culture, 
| iS bt See ego Pees a does. Mp Ai po oa Luk. ape I Herzog (large). | 
| 1 | Saomeomee he MEME do -+---|.. Sosa ee Na | Kokubo. | 
Sere ey ee ee, ee Te ae Ce aR ‘ Pe RE 
nee , aad SE ee = id 6 aot Jk ee eek 3 
