~~ 2 ws he 
These monkeys were kept under observation during fifty-two days. 
They never developed fever, paralysis, swelling of the lower extremities, 
difficulty of respiration, nor any of the other symptoms of beriberi. They 
were, in fact, never otherwise than well, they ate normally during this 
period, and were discharged from observation on January 12, 1906. 
Second series —On March 15, 1906, twelve monkeys (species Macacus 
cynomolgus) were inoculated with the 6 stems, each animal receiving 
intraperitoneally 2 cubic centimeters of a 9-day-old bouillon culture. 
The animals were of both sexes, and included young and full-grown 
individuals. | 
Animal number, Amount. Stem. oa 
Monkey No.— €. ¢. 
se SSE eR TR oe ten Gann amsiamiepeosaae~ 2 Kokubo. 
De fie oboe aN Uh eb soe iwi pn aes ae 2 Do. 
ae ee ee i Pie > ROA CR SR ea a fe 2 Okata I. 
ae te ee oe ad oe mance aoe eee nee ek 2 Do. 
0) RRL AOS A ea a ORR RD A Nae ad ae 2 /| Okata IL. ' 
URIS Riimiicdntindd apace tung wap ane kh Ses e enn ie ERE ae 2 Do. 
POG SS OR SIS Rc 8” en 2 Okata II. | 
BR ees Soak cep eA gE cn tobe teeta te 2 Do. 
| TORE... igi ikc San Seda enke itis Dhan indedeeehn~ so z Herzog I. 
| Dione asic ens Dake, Saab cpoietasanedae anes Z Do. 
| | Eterm SINC LR Se oe ne enna RN 2 Herzog (large). 
| BE asim ies rales elih ieee ae Sahn ier oan Shen Some 2 Do. 
These animals were kept under observation for over one month. They 
remained perfectly well during this time. 
Third series—Twelve half-grown guinea pigs were inoculated on 
February 26, 1906, as follows: Three standard oesen of a 6-day-agar 
culture were suspended in 10 cubic centimeters of physiological salt solu- 
tion. Each guinea pig received 1 cubic centimeter of this suspension 
intraperitoneally or subeutaneously, as shown by the following table: 
Animal number, Amount. Stem. Inoculation. 
Standard 
Guinea pig No.— oese, 
|, EE 5 ee ee? 0.3 OND Too ee ee Intraperitoneally. 
Tse ech oiSey aca = 2 aed a eats. > Subcutaneously. 
| > OSS eee ee 38 Cmte TT osc ae Intraperitoneally. 
PN aia Sen hits wile SS PMs eee BO. cepabbascbaninncce cone Subcutaneously, 
Ns blastp siccacdone 3 Okete- tio oss se ee Intraperitoneally. 
i ae ree ee rE Mpa Beran DD: Sania teene since melee Subcutaneously. 
+1) Pale epee oe 3 HORM 22hco ew ccs- 22-3. ee Intraperitoneally. 
1 ae aera Se - egaey eee Uy Jie ape ey Subcutaneously. 
+? Se ae Be 3 MG TEOR Tice tie diocese ~s>5>-3be Intraperitoneally, 
MeO a oo Sana pana Bi Ty 5 OO) cdibeboawes 2 tte tea Subcutaneously. 
BED. gocin Noa wooae des 3 Hetrog (latte) nci.~ =-<-- a0 Intraperitoneally, 
WARY pilineneesee duekok Bi Nenad G0 dca hack Booted Subcutaneously. 
