2 eee, oe eee ru 
725 
The animals were kept under observation for nearly two months. They 
remained well and did not develop any disease. 
Fourth series—On March 15, 1906, twelve young guinea pigs were 
inoculated intraperitoneally, each with 1 cubic centimeter of a 9-day-old 
bouillon culture of the different stems, as follows 
| Animal number, Amount. Stem. 
pe eg es Be = a 7 ac = 
| Guinea pig No.— C.¢. 
115 Sabai penetra Allan eas PN a ae ee ee i eed 
| 1-2) Cait DIO ce te Oe Oe 1 Do 
LUD oe oe ee oe Sees Rania eea oes a es ON 1 Okata I. 
‘A SR a a a 1 Do. 
| Ef panes RR a fees on) Orme re pA el a Okata II. | 
yD = AR og uo Ge ea Oe ee ee IC ee ee 1 Do. 
SR Sos cans nee 2 Le re 1 Okata III. 
en ia te a CRON 1 | Do. 
1 1 LO Reise on ee ee RR RS RR 1 eris 
RU oe ene bende oun 22 oc tae ae ee 1 = 
LURE ee ecco il ner (large). 
AUS ae etnies Sao os Ee iL op 
These animals were kept under observation for over one month. ‘They 
remained well. 
Fifth series—On March 15, 1906, twenty-four young rabbits were 
inoculated, each receiving intraperitoneally 1 cubic centimeter of a 
9-day-old bouillon culture of one of the 6 stems. 
Animal number. | Amount. ont ; Stem. | 
: te ' a i 6, Toe Lig 
| Rabbits Nos.— p.6: 
| 1869-1872 22 oe So ncn ec Lode ee 1 | Kokubo. 
UNTO hec tats ores acd casas Ae | 1 | Okata I. | 
|. ¢y 2a |_| Ae se eae eee A A | 1 | Okata II. 
TSGIMTSE Sow, sce cot ee cc a ek | 1 Okata III. 
1885-1988 oa sad es oo ee 1 Herzog I. | 
fe.) ee ne ne deh wet ae ee | 1 
| Herzog (large). 
The twenty-four animals were kept under observation for over one 
month. They remained perfectly well. 
Siath sertes—On March 16, 1906, twenty-one rats (mostly of the 
species Mus decumanus but also a number of M. rattus) were inoculated 
intraperitoneally, each receiving a 9-day-old bouillon culture, as follows: 
Rats Nos. 1848 to 1859 each 1 cubic centimeter of stem Kokubo, and rats 
Nos. 1860 to 1868 each the same amount of Okata No. IT. 
March 22, a. m. Rat No. 1848 died during the previous night. Body not 
yet putrid, very much emaciated, On section a grayish white floccule, the size 
of a millet seed, was found on the surface of a loop of the small intestine; 
44510 
— a eT; = i 
