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otherwise no pathological changes were observed. The floccule was composed 
of leucocytes and endothelial cells, and contained innumerable, small diplobacilli. 
Cultures were made from this floeeule and from the spleen and heart’s blood. 
The tubes inoculated from the floccule developed a small bacillus, but no cocci. 
The small intestine of this animal had evidently been wounded during the 
intraperitoneal inoculation. The cultures from the spleen and heart’s blood 
were negative. 
March 27, 1906. Rat No. 1849 was found dead in the morning. Body much 
emaciated, already decidedly putrid. On post-mortem examination there was 
found a caseous abscess the size of a very small hazelnut in the anterior body 
wall opposite the lower ribs of the left side. This abscess contained many 
slender, non-acid proof bacilli. Tubercle bacilli were not found. 
The following rats died subsequently: No. 1860, March 27; No. 1861, March 27; 
No. 1863, March 28; No. 1856, April 7; No. 1859, April 10. Smears from 
the different organs and inoculations of culture media were made from each 
animal. In no case was it possible to regain from the tissues the cocci which 
were inoculated on March 15, and there appears to be no doubt that the inoculated 
organism had nothing to do with the death of the experimental animals. It 
may be stated that a number of the laboratory rats not inoculated but living 
under the same conditions, died at about this time. The other animals of this 
series inoculated with the kakke coccus remained alive; none of them developed 
any symptoms of beriberi. 
BLOOD EXAMINATIONS OF BERIBERI CASES IN MANILA. 
Blood examinations in twelve cases of beriberi were made by the author 
during the years 1904 and 1905 with the object of obtaining the specific 
micro-organism of the disease from the blood, if it was present therein. 
These examinations gave a negative result and no detailed record of them 
has been preserved. Since my return from Japan, over forty additional 
blood examinations have been made, and detailed records of these have 
been kept. On December 7 and 9, 1905, 39 male inmates of Hospital B 
of Bilibid Prison, who were suffering at this time with all types of 
beriberi except that of the most acute, pernicious variety, were examined. 
Some of the cases were of the wet, hypertrophic type, others of the dry 
and atrophic variety ; in certain ones all of the more prominent symptoms 
of beriberi were still present; in others, convalescence was well advanced. 
These blood examinations were undertaken with the assistance of Mr. 
Charles B. Hare, at that time a member of the staff of the Biological 
Laboratory. 
The method of procedure was as follows: 
The skin over the median cephalic vein of the elbow was cleansed with soap 
and water, bichloride solution, and aleohol. The vein was then pierced with a 
hypodermic syringe needle and from 1 to 2 cubic centimeters of blood withdrawn 
and at once injected into a 100-cubic-centimeter flask containing 50 cubie cen- 
timeters of slightly alkaline bouillon. The cultures were then incubated at 37° 
and kept under observation for several weeks. 
