727 
Thirty-three of the flasks remained permanently sterile. In SIX, a 
growth developed in which the following organisms were isolated.* 
s 8 
Culture No. 2 developed a coceus, No. 4 a staphylococcus, No. 13 a large 
staphylococcus, No. 19 a spore-bearing bacillus, No. 26 a large diplocoecus, atid 
No. 37 a medium-sized, slender, spore-forming bacillus. All of the above stained 
well by Gram’s method. 
The four varieties of cocci which were obtained have not as yet been 
very extensively examined as to their cultural properties. However, 
none of them seem to be identical with the cocci brought from Japan, 
and experiments made with them with the object of producing beriberi in 
animals have all been negative, as will be seen from the following table: 
Seventh series.—Twelve monkeys, each received intraperitoneally one-fifth of 
an oese of an agar culture in 1 cubic centimeter of physiological salt solution, as 
follows: 
Organism isolated Organism isolated 
from Bilibid from Bilibid 
Monkey No.— __ beriberi case No.— Monkey No.— _ beriberi case No.— 
1655 2 1661 13 
1656 Ys 1662 ° 13 
1657 4 1663 26 
1658 4 1664 26 
1659 19 1665 37 
1660 19 1666 37 
These monkeys were well during the days following the injections and 
they neither developed symptoms of beriberi nor manifested appearances 
of any disease. 
In a case of hypertrophic beriberi,?? which quickly terminated fatally, 
a blood examination was made January 26, 1906, five days before death. 
(See necrospy No. 1636, page 747 of this article.) At this time the 
patient presented all of the symptoms of an acute and profound case of 
beriberi. The examination included anaérobic cultures. 
About 5 cubic centimeters of blood were withdrawn from the median cephalic 
vein. Two flasks containing 50 cubie centimeters of bouillon were inoculated, 
each with 1 cubic centimeter of blood. One was kept aérobically, and the other 
in a hydrogen atmosphere, anaérobically. Several agar tubes were likewise inoc- 
ulated from the blood, some of which were also developed aérobically, and some 
anaérobically (Buchner’s pyrogallic acid method being used). The cultures were 
left under observation during from four to five weeks. They remained per- 
manently sterile. 
Apparently, all of the cultures from this case had remained sterile, 
but the possibility was considered that perhaps something ultramicroscopic 
in character and which did not give rise to any changes in the culture 
media, detectable by either ordinary macroscopic or microscopic examina- 
“The cultures are designated by the same numbers under which the 39 cases 
of beriberi were recorded. 
“ Occurring in the Civil Hospital. 
