728 
tions, might have developed. Hence, 6 monkeys were inoculated from 
the culture media, which had been kept both aérobically and anaérobically. 
Bighth series.— 
Animal number. Date. Amount. Culture. 
Monkey No.— | i 0, 
yi & Ge een eR a emer ae Se eee aee Feb. 2, 1906 | 1 Aérobie culture A. 
AD a te te ee ee ete cl ae 2 dot... | sf Anaérobie culture A. 
eR ie ee cc paeeas | 1 Aérobie culture B. 
ee Sa ee ee ee oe et G6 420-0 1 Anaérobic culture B,. 
be a ae Ent oe es, Se eS EP ea dot. | 1 Aérobie culture D. 
VTE dD cvuccceeds cectuunamadenctubaaans= Somerset (: (3 ea | 1 Anaérobie culture D. 
These animals never showed any sign of disease and remained per- 
manently well. 
Before the bouillon cultures from the above case of beriberi were used 
for inoculating the animals recorded in the preceding tables, subcultures 
from the tubes were made on agar. These were preserved in the incu- 
bator both under aérobic and anaérobie conditions. After four weeks 
it was noticed that each of two of the tubes had developed a single colony. 
The one which had been kept under anaérobic conditions contained 
large, yellow cocci, which later also grew aérobically; the aérobie tube 
showed a dirty, grayish-brown, very tenacious colony composed of bacilli, 
which stained very irregularly, and appeared morphologically somewhat 
like diphtheria bacilli from an older culture. After transplanting a 
portion of this growth to a fresh agar tube the remainder of the colony 
was rubbed up with 15 cubie centimeters of sterile salt solution; with 
this very imperfect suspension, which contained comparatively large 
floceuli, six monkeys were inoculated, each with about 1.3 cubic centi- 
meters of fluid. 
Ninth series.— 
Animal number. Date. — 
Monkey No.— | e:°; 
I a ee deen nena oe cea caaeagew ean dd aeanne ames Mar. 7, 1906 1.30 
GN en ee nea bane me aoe aad Suro neck wenanam ee We Se 2s, aay eee | 1.30 
EEE Se ea ae EEN CRE ARE I rte rn em mah oe | 1,80 
ee kc cea pada Weawoncanasendccuwencanh Serene seeelelameate do _- ee | 1,30 
ea 0k OTE UR al Sk ta a ea pene 1.30 | 
The animals remained permanently well. 
Cultures were made from the internal organs in a number of post- 
mortem examinations of beriberi cases and examined with the view of 
ascertaining whether the identical coccus brought from Japan was present. 
Most of these were negative. An acute case of beriberi died at Bilibid 
