910 
V. ParHogENrsts oF AmMa@Ba!—Continued. 
2. Original work. 
(a) Experimental ameebic colitis. 
(b) Experimental ameebic abscesses of liver. 
(c) Experimental ameebic abscess of the omentum, spleen, lung, 
ete. 
(d) Experimental ameebic septicaemia. 
(e) Immunity. 
(f) Ameebic lesions (how produced). 
VI. Summary. 
I. INTRODUCTION. 
Since our first publication in 1904, on the cultivation and etiologic 
significance of amcebe,* a number of articles dealing with the subject 
have appeared which in the main have been confirmatory of our results. 
Our own work has been continued and, in addition to confirming the 
observations given in the first paper, enough new facts have been estab- 
lished to make further publication at this time desirable. As we have 
absolute control of the large clinical and pathological material of St. 
Paul’s Hospital and free access to that of Bilibid Prison Hospital as well 
as to numerous other less important sources, our opportunities have been 
all that could be desired. 
In studying the more purely biological problems connected with this 
subject, we have felt the lack of advice and association with a trained 
zoologist, and for that reason have avoided discussing much that is 
interesting and definite in the study of cultures of amcebe. However, 
there are a number of points in this connection which have clearly been 
established, and, notwithstanding the fact that the results differ in certain 
instances from the expressed views of some eminent zodlogists, they are 
given with full conviction of their accuracy, together with the experi- 
mental proof. 
The most important question dealt with in this paper (and it seems 
to us that this is the keystone to the whole problem of ameebic infection) 
is the property, possessed by apparently saprophytic ameebe from the 
outside world, by virtue of which the amebe become so modified by 
successive stages and changes in their environment or by symbioses that 
they become true parasites for monkeys, guinea pigs, and human beings. 
Our experiments demostrate beyond doubt that this is the property of 
many amcebe and this fact makes clear much which has formerly been 
confused about the pathogenesis of amoebee. 
As much of this work is so closely connected with the observations and 
conclusions given in our original paper, it is suggested that the reader 
consider that article in connection with this one. 
tAmebas: Their Cultivation and Etiologic Significance, by W. E. Musgrave, 
M. D., and Moses T. Clegg, Publications of the Bureau of Government Labora- 
tories, Biological Laboratory, Manila (1904), No. 18. 
