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of our most recent cultures isolated from vegetables purchased in a 
Manila market, we have produced liver abscesses in monkeys after intra- 
peritoneal inoculation. 
ORIGINAL WORK. 
In our first publication conclusive evidence was given of the pathogenic 
character of amoebe in producing ulcerative colitis in man and monkeys. 
We now wish to offer more evidence upon this point and in addition to 
show that the amoeba is capable of a very definite parasitism. Further- 
more, we wish to demonstrate that morphologically the organisms which 
produce this colitis may resemble either “A. coli” or “E. histolytica” as 
they are described by Schaudinn and that they may be. isolated from a 
variety of sources. It is almost impossible to conceive of man ever before 
having been in any way associated with some of these sources. 
We have used cultures of encysted ameebx in all of our experiments, 
because we have long been convinced that these are the most certain to 
be infectious. 
INTESTINAL INFECTION, 
SUMMARY OF EXPERIMENTS.11 
(1) If ameebe from any source are cultivated on artificial media, and 
the encysted cultures are then fed to monkeys by means of a stomach 
tube, after a variable time the stools of some of the animals will contain 
the organisms. The usual symptoms of infection are observed, and at 
autopsy the lesions and parasites characteristic of amoebiasis will be 
found. However, in the cases of many of these animals no bad results 
follow the introduction of the organisms and amoebe are never found 
in the stools. In others, after the infection is established, the animal 
may finally entirely recover and the amoebee disappear. 
In still another class, ameebe after a certain incubation period, may be 
found for a time, finally to disappear from the stools without any visible 
symptoms of infection. However, if the animals are killed, in some 
of them ameebic lesions will be found in the colon. One monkey which 
was infected in the manner detailed aboye developed on ameebic abscess 
of the liver which was found at autopsy, and in another an abscess of the 
lung was observed. 
The percentage of infections to be included in this class of experiments 
does not appear to be materially inflenced by the type of the symbiotic 
bacterium which was present in the cultures, nor are the results very 
different when the amcebe are in symbiosis with more than one variety 
of bacteria. If large doses of the symbiotic bacteria alone are first. fed 
to the animals for several days and if then the culture of the ama@be and 
the bacteria is ingested, the percentage of infections is increased. 
™See also Publications of the Bureau of Government Laboratories, Manila 
(1905), No. 18, 70-76. 
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