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ILLUSTRATIONS. 
In figs. Nos. 1 to 9, inclusive, the magnification in all is exactly 800 diameters. 
The reader is requested while examining these reproductions to bear in mind 
the characteristics which have been designated as distinguishing between “H. coli” 
and “EH. histolytica.” 
Figs. Nos. 1 to 3, inclusive, photographs of ameba “y.” This ameba was 
grown from vegetables and is a pathogenic parasite. With cultures of it we 
have produced amoebic colitis and amcebic abscesses in the liver, lungs, omen- 
tum and spleen of animals. 
No. 1. Forty-eight-hour transplant, inoculated from an old, encysted culture. 
No, 2. Twenty-four-hour transplant from No. 1. 
No. 3. Twenty-four-hour transplant from No. 2. 
The amplification in these three pictures is exactly the same (800) and 
shows the variation in size in a pure species of ameba. This is quite decided 
between the encysted forms in No. | und the vegetative form in No. 3. In 
No. 1 are shown fully developed cysts, others in which reproductive changes 
are appearing, and several small vegetative forms which have escaped from 
cysts. Eneysted amebe may also be seen, showing the rather dense, prominent 
ectosare sharply distinguishable from the endoplasm as in Schaudinn’s “F. his- 
tolytica” but, on the other hand, in the same amebe the prominent central 
nucleus with a distinct nuclear membrane, as in “HE. coli,” may also be seen. 
Early reproductive changes are shown in several of the ameebe, and in these the 
line of demarcation between ecto- and endo-plasm is less marked. No. 2 shows a 
cyst containing a single young ameba and another ruptured cyst from which an 
amoeba is about to escape. This phenomenon was also shown in figs. 29 to 32 in 
our first report. No. 3 represents a vegetative form, probably a young but full 
grown parasite; except in the large pseudopod which is thrown out, the distine- 
tion between ecto- and endo-plasm is not sharp, and the nucleus accredited to 
“H. coli” is also present. 
Figs. Nos. 4 to 6, inclusive, photographs of amoeba “cabbage.” This ameba was 
grown from cabbage and is a very pathogenic parasite. With cultures of it 
any of the lesions described in this paper may be produced. 
No, 4. Forty-eight-hour-old transplant, showing young vegetative forms and 
an early cyst. 
No. 5. Twenty-four-hour transplant from an old, eneysted culture, showing 
four old cysts and two bunches of young ameebe freshly liberated. 
No. 6. Vegetative form of figs. 4 and 5. The ectoplasm is prominent in the 
encysted forms in figs. 4 and 5, and in the fresh cyst in fig. 4 the 
differentation from endoplasm is distinct. None of these amb 
show a nucleus. In the vegetative form of the same ameeba in fig. 6 
we have the prominent nucleus described for “H. coli” as well as the 
lack of differentiation between ecto- and endo-plasm. 
949 
ay 
