; 
ee ee ee 
— see oe, Ve 
owe vitae, Pa, ee te eee Pe ee eee ey ee ee | ee 
e 
i+ Faae = 
ang iad WEY i a Nk, i oT 
ee ae he 
PRT ae a eS eee ee 
964 
TaBLE VII.—Agglutination of the fifteen types of dysentery bacilli with dysenteric 
immune serum prepared from several strains—Continued., 
Rabbits’ immune serum, eo Wives 
Byne| . Ongantem, | 'inuone [7 ——— re 
“Port fy | “Korea “Tokyo “Shiga.” “Rio- - “Shiga.” 
Arthur. iit. IV. yang I. 
Got PGko lcci: 1-80 1-500 | 1-7,500 1-750 1-750 1-800 
H Ores eu. 22 1-600 | 1-2,000 | 1-1, 000 1-1,000 | 1-2,000 | 1-2, 000 
I Rio-yang I______- 1-80 1-800 1-750 1-3,000 | 1-5,000 | 1-1, 000 
a pe 0 1-200 | 1-300 | 1-5,000 1-100 1-800 1-500 
K Toky? IT. <..=. AO Acs. 1-100 | 1-2, 000 1-750 1-200 | 1-1,000 | 1-2, 000 
L Yamaguchi-______ 1-200 | 1-2, 000 1-750 1-750 | 1-5,000 | 1-2,000 
AES | TORVOT Voice! 1-20 1-300 | 1-5, 000 1-500 1-750 1-500 
WN) Boreas) 1-200 | 1-2, 000 1-500 1-2,000 | 1-5, 000'| 1-2, 000 
0 MeV ORO A oi 2. 1-100 | 1-1,000 | 1-2,000 1-7, 500 1-100 | 1-2, 000 
In Table VII we see the high agglutination of “Korea Il” (an acid 
strain) produced by dysenteric immune serum prepared with ‘Port 
Arthur” culture (a non-acid strain). Vice versa a similar result in 
agglutination was obtained with dysenteric immune serum prepared with 
“Korea IIT” (an acid strain) on “Rio-yang II” (a non-acid strain). 
It may also be seen that immune serum “Tokyo IV” (an acid strain) 
has the power to agglutinate “Rio-yang II” and “Wakayama I” (non- 
acid strains) in very high dilutions even with dysenteric-immune rabbit 
serum, which is designated as a “differentiating serum” by German 
authors. With the immune serum of goats and of horses, as Shiga and 
some German authors have reported, there is very little difference in the 
agglutinative reactions between both the acid and the non-acid types. 
In addition, a study of Table VIT will reveal the results of the agglutina- 
tion tests of all fifteen of the strains of dysentery bacilli with immune 
serum of rabbits, goats, and horses; by a glance it may at once be seen, 
that there is no apparent distinction in agglutinative reactions between 
the so-called mannite fermenting and non-fermenting organisms and 
that no such distinction can correctly be made. 
STUDIES ON BACTERIOLYSIS. 
Bacteriolysis by immune serum being an important method in the 
identification and differentiation of bacterial species, it seems strange to 
me that most authors who have studied the differentiation of the types 
a sil Pee Ne 
vig 
a 
