eee ee we ee on ee 
970 
From all these experiments we can conclude that bacteriolysis of the 
different strains in vitro may take place irrespective of the type, 
whether acid or non-acid, and also irrespective of whether the immune 
serum has been prepared with an acid or non-acid strain. On the basis 
of these facts, and in view of the results of the fermentation and ag- 
glutination tests, I must again emphasize that I can see no reason what- 
ever for recognizing two distinct groups of bacilli as suggested by Lentz, 
namely, those which ferment mannite and those which do not, nor for 
distinguishing between the so-called “dysentery” and “pseudo-dysentery” 
bacilli as proposed by Kruse. Furthermore, these results are in conform- 
ity with those of Flexner, Strong, Gay, and other American authors, 
in showing that both types of dysentery bacilli have equal etiological im- 
portance and that the differences between them are unimportant. 
In the preceding pages I have demonstrated that there are fifteen 
varieties of dysentery bacilli, nine of which ferment mannite and six 
of which do not. TI have also proved that the separation of these varieties 
into two groups according to their power of fermenting mannite can not 
be made in conformity with their agglutinative and bacteriolytic reac- 
tions, and, in addition, I have endeavored to test the comparative aggluti- 
native and bacteriolytic reactions of bacilli which have the same prop- 
erties in regard to fermentation. Among the seventy-four strains of 
dysentery bacilli which have been isolated in Japan, Korea, Manchuria, 
from the Japanese and Baltic fleets, and in the Philippine Islands, four 
are of the “A” type (“Shiga”), six of the “L” type (“Yamaguchi”), 
and seven of the “I” type (“Rio-yang I”). As Tables XIII, XIV, and 
XV show, the several strains of the same type do not possess homologous 
agglutinative and bacteriolytic reactions against the homologous anti- 
dysenteric serum, 
Taste XILI.—Agglutination and bacteriolysis of bacilli of the same type with “Shiga” 
(‘‘A’’) with the same sera. 
Bacilli tested. _Port Ar-| “Gaiping | wahiga.” | Cine IP? 
Anti-dysenteric serum (rabbit serum): 
“Shiga” (‘‘A’”’ type, non-acid)— 
Agglutination --..-------------- -------- ---- 600 600 1, 000 600 
Bacteriolysis _-....-------------------------- — 0. 005 0. 001 — 
“Kyoto” ('O” type, acid)— 
Agalutination ....:.--.--—--------------<<-- —(1-20) | —(1-20) 75 | 200 
Bacteriolysies +. .- 32. ---.----+4-- set cee 0.01 0. 005 0. 001 | _ 
