973 
TaBLeE X VIII.—Cross bacteriolysis of six types of dysentery bacilli tested 
with homologous sera. 
Non-acid bacilli. Acid bacilli. 
Sera and type. . 
st hot? LNB Pi asap 7? “ug ‘oH? i ld 
Non-acid sera: | 
| 
| 
AY nmmmnnnannnnn ial +/+ ]/—/]—|] + /] + 
“D” _-_-.-.------------ 1 beeen ate” 4h + 
See ee eae ee _ — | + + + — 
Acid sera; | 
i SE —|/+i+)¢]4+i]-— 
eee aceon eee i rae eS ea +}/—] + 
amma Tis | oe |e 
SUMMARY AND CONCLUSIONS. 
In order that complete observations might be obtained, 74 strains of 
dysentery bacilli were isolated, collected, and identified from several 
sources, Japan, Korea, Manchuria, the Philippine Islands, and the 
Japanese and Baltic fleets (during the Russo-Japanese war). Upon a 
comparison of all these strains it was found that they could not be 
distinguished from one another either by their morphological or cultural 
properties, although minor differences were shown to exist in their fer- 
mentative powers and their reaction toward immune serum. 
In my experiments I was not satisfied in attempting a differentiation 
of the different types of bacilli, based on the fermentation and agglutina- 
tion reactions alone, but in addition their bacteriolytic action toward 
immune sera was employed. 
(1) The results of the study indicate that the great majority of the 
bacilli which have been isolated during the past few years from cases of 
acute dysentery (not due to amcebe) must be considered as being the 
exciting factor of the disturbance. The organisms which have come into 
my possession and which have been isolated as the cause of dysentery may 
be separated into fifteen groups which have fermentative characters dis- 
tinguishing them one from the other, six not fermenting, and nine 
fermenting mannite (so called non-acid and acid bacilli.) 
(2) The mannite fermenting types are widely scattered over the world 
and certainly cause characteristic sporadic cases and epidemics of dysen- 
tery. The form of the disease caused by them is often severe. On the 
other hand, the non-fermenting ones often give rise to milder cases of 
infection (as in Manchuria). 
(3) The grouping of the different organisms, according to the dif- 
ferences in their powers of causing fermentation, does not correspond to 
that which results from differences observed in agglutinative and bac- 
teriolytic action with specific immune sera. The anti-dysenteric rabbit 
480405 
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