90 The Philippine Journal of Science 1922 
Two species of Volvox proper that occur in my Philippine 
material more or less mixed with species of the related genera 
I have described in another paper (Shaw, ’22) in which a special 
genus was made for the European species long known as V. 
aureus Ehrenberg. 
MERRILLOSPHAERA CARTERI VAR. MANILANA VAR, NOV. 
DESCRIPTION OF THE TYPE SPECIMEN 
For the type of this variety, an asexual specimen (Plate 1, 
figs. 1 and 2) has been selected in order to exhibit a character 
which is essential to distinguish the genus from Campbello- 
sphaera. This character appears in embryos that are in a stage 
which makes it fairly plain that the gonidia are differentiated 
early, but do not migrate. 
The specimen (No. 1) is one of a batch collected from Pond 
C in Pasay, near the southern outskirts of Manila, September 
20, 1914, and fixed at some time on the next day in picro- 
nigrosin. Material from this batch was mounted in glycerine on 
several slides and sealed with shellac. Slide 4 of this lot bears 
the specimen under a cover glass with glass rodlets measuring 
309 to 354 yw, which were distributed for cover supports but 
are loose and now clustered at one side of the circle. The gly- 
cerine is strongly colored with picric acid. There is no record of 
the time when the mounts were made, but the photographs were 
taken June 13, 1916. So they were at the time of making 
measurements (July, 1919) more than three years old. The 
mount had then taken up no air bubble,' but the paper label 
of the slide showed glycerine on the edge nearest the cover, which 
raised a suspicion as to the security of the sealing. The slide 
bears a mixture of several species of Volvoceae. There are nu- 
merous hyphae extending into and through the glycerine; they 
appear to have developed in it. 
The type specimen measures 545 by 590 ». The upper and 
lower sides are seen with a difference of focal adjustment 
amounting to about 250 », which, with an allowance of 1.4 for 
the optical density of the glycerine, gives an estimated thickness 
for the specimen of about 350 ». The area of the somatic 
surface that is flattened by the cover glass is about 350 by 400 » 
in extent. Within this area the somatic protoplasts of the 
*The specimen has since been ruined, partly by escape of glycerine and 
formation of a large bubble, and partly by destructive movement of glass 
rodlets. 
