21,2 Shaw: Copelandosphaera 209 
The specimen is a globose coenobium containing nine daughters 
that fill the middle and hindmost thirds of the mother and 
cause the wall of the rear half of the mother to bulge out into 
the form of large, closely set bosses.? The specimen is com- 
pressed under the cover glass to about 400 » and measures 1,050 mn 
wide and 1,015 » long. The number of: cells in the mother 
was estimated at 52,600, the average spacing of the protoplasts 
being about 8.5 ». The front of the coenobium is turned away 
from the observer about 25°. 
The somatic protoplasts are ovoid or ellipsoidal. In the front 
of the coenobium they measure about 5 by 8». At the sides and 
back they are somewhat smaller. The thickness of the space 
occupied by the layer of somatic cells with their membranes 
is about 14 to 19 » in different parts of the front, and about 
10 » at the back over the daughters. 
The nine daughter coenobia are arranged in a way that leaves 
one vacant space in the back part of the group. Four daughters 
that lie in the median optical section of the mother press the 
wall of the latter out to form bosses that are of a height equal 
to one quarter or more of the diameters of the daughters. The 
daughters are all more or less ellipsoidal, the smallest measur- 
ing 275 by 315 » and the largest 315 by 365 », the average 
dimensions being 298 by 322 ». An estimate of the number 
‘of cells in the smallest daughter gave 15,000, the protoplasts 
being about 2.5 » in diameter and the spaces between about 1 x. 
All of the daughters are asexual. The largest contains ten 
gonidia of about 21 » diameter. The smallest contains 2-celled 
embryos of about 21 » diameter and 4-celled embryos of about 
22 » diameter. The largest embryos are 8-celled ones measur- 
ing 30 yw. The numbers of the reproductive bodies in the 
daughters are: 
6, 7, 8, 9, and 10 reproductive bodies in 
2, 2, 3, 1, and 1 daughters, respectively. 
The most-advanced embryos, 8-celled, are in the four daughters 
that are nearest the front of the mother. 
The thickness of the layer of somatic cells of a daughter with 
their membranes can be made out by direct microscopic obser- 
vation of the daughter that bulges out on the right side of the 
mother. There is a line parallel with the peripheral boundary 
of the daughter and about 10 to 12 below it. This is shown in 
*This bossed condition of the mature asexual coenobium is partly, if 
not wholly, a result of the manner in which the shrinkage produced by the 
mounting medium has taken place. 
