220 The Philippine Journal of Science 1922 
on the cell membranes, Powers received the impression that 
these also resemble those of V. tertius. 
A well-developed stigma in each of the somatic cells about 
the anterior pole of the coenobium was observed by Powers (708, 
p. 150), and in the cells farther from that pole the stigmata 
were proportionately smaller, until a little back of the coenobial 
equator they were no longer visible as colored bodies. Powers 
was unable to decide whether at the posterior pole they were 
really quite absent, or whether one of a group of small colorless 
granules was the homologue of a pigment body. No recogniz- 
able stigma could be seen about the posterior pole even with 
an immersion lens. The stigmata about the anterior poles were 
visible in the daughters before birth. 
Reproductive cells of one, two, or three kinds occur, accord- 
ing to Powers, in the same coenobium in numbers ranging from 
one to twenty-five, the numbers being about the same regard- 
less of what kind or kinds were present. These reproductive 
cells are evidently differentiated at about the 256-celled stage of 
the development of the daughter coenobium. That such is the 
case appears from a comparison of the reproductive cells of a 
daughter coenobium with the cells of a sperm sphere found in 
the same mother (Powers ’07, pl. 13, figs. 13 and 14). This 
sperm sphere consists of about 256 roundish cells, the maximum 
number for such a structure. The reproductive cells in the 
neighboring daughter coenobium are very similar in size and 
shape to these sperm-sphere cells, a fact which indicates that 
they were formed at the 256-celled stage and remained undivided 
while the somatogenic cells divided three times, producing about 
1,800 somatic cells. 
The number of reproductive cells in the daughter coenobia 
within four mother coenobia was determined by Powers (’07, 
p. 134) and they were found to occur with frequencies as given 
in Table 2. 
TABLE 2.—Distribution of reproductive cells in the daughters contained 
in four mother coenobia of Copelandosphaera spermatosphaera. 
Number of reproductive cells. 
Number of daughters. 
15 | 16 | 17 | 18 | 19 | 20 | 21 | of | 28 | 24 
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