326 The Philippine Journal of Science 1920 
was found very helpful to the present writer’s problems, but 
listed results with only such disinfectants as mercuric bichloride, 
lysol, formaldehyde, etc.; Bordeaux mixtures, lime sulphur, and 
Burgundy mixtures were not reported upon. Data upon these 
last-mentioned fungicidés were expected to be of value in our 
control problems, and tests with them were therefore begun. 
The method of Anderson and McClintic seemed to be the simplest 
and most desirable for this work. The following is a presenta- 
tion of data obtained from these experiments: 
BRIEF RESUME OF METHODS OF ANDERSON AND MCCLINTIC 
Methods were devised by these investigators to test disinfect- 
ants both in the presence and in the absence of organic matter, 
using a standardized culture of Bacillus typhosus. In the work 
reported in the present paper, Pseudomonas citri of course was 
used for the tests. Since the choice of the organic matter in 
their methods was entirely empirical, it would seem that the 
tests in the absence of organic matter were most desirable for 
this problem. In either case the results in the present tests are 
for the most part comparative and cannot be considered as 
directly applicable. 
The tests without organic material were made by exposing a 
carefully measured volume of a standardized culture of Pseu- 
domonas citri to various dilutions of the disinfectants. Each 
dilution of each disinfectant was tested for periods of 24, 5, 
74, 10, 124, and 15 minutes. The standardized culture in our 
experiments was a 3-day-old culture of Pseudomonas citri which 
had been previously transferred successively from 3-day-old 
_ bouillon cultures. The bouillon used was the standard nutrient 
beef peptone bouillon +-1.5. 
The culture to be used for the tests was first cooled to 20° C., 
and by means of sterile pipettes 0.1 cubic centimeter of this 
culture was then added to each of ten tubes, each containing 5 
cubic centimeters of the dilutions of fungicides to be tested ; these 
dilutions were also cooled and maintained at 20° C. The seeding 
tubes were then rotated to distribute the organisms throughout 
the disinfectant, and at the end of the periods of exposure one 
loopful of the mixed disinfectant and culture was removed by 
means of carefully standardized 3-millimeter platinum loops, 
flamed of course. The loopful of inoculated fungicide was then 
immediately transferred to a tube of nutrient beef bouillon 
1.5; the killing of the canker bacteria was indicated by the 
absence of clouding in the bouillon tube. The presence of cloud- 
