386 The Philippine Journal of Science 1920 
actively motile amcebe. On forcibly opening the adherent coils 
of the intestine, a large collection of creamy pus was found 
inside the cavity formed by the adherent coils of the intestine. 
The pus cavity was found completely surrounded by the coils; 
no opening joining the cavity with the lumen of the intestine 
could be discovered. Smears of the pus showed numerous ac- 
tively motile amcebe. On staining the smears the pus was 
found to be free from other microérganisms. Several stained 
preparations were made from this pus. Another peculiarity 
found in the case was the presence of dysenteric lesions in the 
small intestine extending about 3 inches above the ileoccecal 
valve. Three typical dysenteric ulcers were found in this area. 
Lastly, an elongated, elevated, inflammatory patch was found on 
the peritoneal surface of the small intestine opposite the ulcers— 
the appearance being similar to that found in the large intestine. 
Smears from this patch showed an abundance of living amcebe, 
while smears from the other portions of the peritoneal surface 
of the intestine showed no amcebe. *A portion of the affected 
small intestine and large intestine were removed for sectioning. 
CHARACTERS OF THE LIVING AMGBA 
In fresh preparations made with pus taken from the pus 
cavity, numerous actively motile amcebe were found. As it was 
not possible to stain preparations at the time that this exam- 
ination was made, it was not suspected at the time that the 
amceba differed altogether from the classical type, and accord- 
ingly no special attempt was made to make out any distinctive 
characters of the living amcebee. All that I remember was that 
the amcebeze showed marked size differences. Some of them were 
vacuolated. The ectoplasmic and endoplasmic differentiation 
was notably marked. 
STAINED PREPARATIONS 
Stained preparations were made by making films, fixing them 
while still wet in acetic acid-picrie acid solution and then stain- 
ing them by a modification of Dobell’s iron-hematein method. 
The slides were stained overnight and then differentiated by 
ferrous alum solution under microscopic control. On cursory 
examination of these slides under the oil-immersion objective 
I noticed that the nucleus of the amceba was not of the karyoso- 
mic type. On carefully examining a large number of individuals 
in the several preparations I made, I found the amebe showed 
the following characters: 
