17, 4 Chatterjee: An Atypical Ameba 389 
the transverse and longitudinal muscular coats of the intestine. 
On examination under high power, it was found to be composed 
of a dense collection of cells interposed among a granular débris. 
Between the small faintly stained cells a few large irregularly 
shaped cells wére found under the oil-immersion lens. These 
were the amebe. No collections of red blood corpuscles, or 
leucocytes, or fibrin threads—so characteristic of inflammatory 
lesions caused by microérganisms—were found. 
Interposed between this faintly stained cellular layer and the 
longitudinal muscular coat was found a layer of tissue that 
was very vascular and which took the stain deeply (Plate 3, b). 
This was evidently the subperitoneal tissue. On its outer bound- 
ary was seen a layer of cells, evidently the endothelial lining of 
the peritoneum. Careful study of this vascular subperitoneal 
layer under the oil-immersion lens showed it to be composed of 
numerous engorged vessels. The intervening tissue outside the 
vessels was full of a granular débris, among which were found 
several large cells. The outlines of these could only be clearly 
distinguished by constantly changing the focus. These cells 
showed a single eccentrically placed nucleus. In some, the nu- 
cleus could not be seen. In some, obscure vacuolation was ob- 
served. They were much larger than the leucocytes. These, I 
have satisfied myself, are the amcebe. On examining the mus- 
cular layer, no abnormality was found except infiltration by gran- 
ulation cells although here and there fragmentation of the muscle 
was encountered. On examining the mucous and submucous 
coats of the small intestine the vessels of the latter were found to 
be very much engorged (Plate 3, f). Numerous collections of 
granulation cells were found, but I failed to discover any cell 
having the appearance of an ameba. In the mucous layer, the 
mucous glands were seen to be embedded in a cellular infiltration. 
The portion of the glands abutting on the lumen was found obli- 
terated and it had been replaced by a cellular infiltration and by 
hemorrhagic patches. On carefully examining the infiltrated 
mucous membrane with the oil-immersion lens, I failed to find 
any cell resembling an amceba except among the hemorrhagic 
patches. In these hemorrhages are found big amoeboid cells 
surrounded by erythrocytes. Plate 2, fig 2, is a representation 
of one of these patches as seen under the oil-immersion lens. 
On studying sections of that portion of the large intestine 
which was found embedded in the abscess cavity, no trace of the 
mucous coat could be distinguished. A considerable portion of 
the muscular coat had been replaced by granulation cells em- 
173259-——5 
