222 



Philippine Journal of Science 



our analytical work on the different brands of tikitiki extract as 

 prepared in local drug stores and private laboratories. However, 

 inspection of the results shows that extracts 1, 2, and 3 are of 

 approximately equal concentration, while extracts 4 and 5 occur 

 as much lower concentrations, this being shown by their specific 

 gravity and the proportions of total solids they contain. The 

 results of our analyses are summarized in Table I. 



Table I. — Results of analyses of samples of tikitiki extract. 





Bureau of 

 Science, 

 extract 1. 



Extract 2. 



Extract 3. 



Extract 4. 



Extract 5. 



Temperature °C.. 



28 to 25 



26 to 25 



30. 3 to 30. 1 



28. 5 to 80. 1 



29. 3 to 29. 6 





1.2800 



1.2960 



1.2878 



1.2254 



0. 9572 



Total solids .per cent.. 



53.23 



56.20 



50.33 



25.53 



5.40 



Keducing sugar (by direct 













reduction) per cent.. 



23.70 



20.80 



24.96 



15.27 



1.39 



Disaccharides (saccharose 













by inversion) . .per cent.. 



2.56 



0.30 



0.65 



0.57 



0.17 



Mineral residue.. ..do 



4.40 



3.32 



4.87 



0.76 



0.19 



Total nitrogen by Kjel- 













dahl per cent.. 



2.008 



2.300 



1.640 



0.856 



0.140 



Phosphorus as P20s.do 



1.70 



0.375 



2.14 



0.24 



0.165 



Sulphur (total) do 



0.26 



0.109 



1.11 



0.059 



0.001 



Amylolytic enzyme 



negative 



negative 



negative 



negative 



negative 



A striking fact in this table is that extract 3, which has a 

 higher specific gravity than extract 1, has however a lower per- 

 centage of total solids than that extract. This fact might be 

 accounted for by the different methods used in removing the 

 alcohol from the extract and by the extent of its dilution with 

 water so as to obtain it at the desired concentration. Further- 

 more, we have the anomalous condition shown in extract 5, in 

 which a sample with 5.4 per cent of total solids shows, at 29.3° 

 to 29.6° C, a specific gravity of 0.9572. This condition might 

 be explained by the presence of an unremoved residuum of the 

 alcohol that was used as a solvent. 



The determination of total solids in vacuum is undoubtedly 

 the most accurate method for collecting such data; but, regret- 

 tably, our electric pump was not in working order at the time 

 we undertook this investigation and we were forced, therefore, 

 to employ other methods to obtain our results. 



The amounts of reducing sugar and disaccharides are practi- 

 cally proportional to the total solids. The proportion between 

 them, however, is variable and depends upon the degree of their 

 hydrolyzation. In extract 2, which was purposely hydrolyzed, 

 the proportion of disaccharides is very small. 



