16, 2 Santos y Alvarez: Copra Meal 183 
hour and then filtered. This treatment removed the water- 
soluble protein and a part of the salt-soluble, together with the 
starch and other soluble carbohydrates. The residue was washed 
thoroughly with hot water. The filtrate together with the wash- 
ing was treated quantitatively for total nitrogen, and the residue 
hydrolyzed with 20 per cent hydrochloric acid(14) for forty-eight 
hours. The distribution of nitrogen in the hydrolysate was de- 
termined according to the directions in Plimmer’s monograph (19) 
with certain modifications, stated below. The residue from hy- 
drolysis was treated quantitatively for hydrochloric acid insoluble 
humin nitrogen in accordance with the method of Gortner and 
Holm. (6) 
TABLE 2.—Distribution of nitrogen in copra meal (two-gram samples). 
Total 
| Nitrogen Different forms of | Nitrogen 
> os itrogen 
in each | Nitrogen grate hie, ae Nitrogen|,™ Neb 
| sample. | average. | pyar en ecm | sample. |A¥€TS2- |"in hot 
water. 
- mg. mg. mg. mg. |Per cent. 
Nitrogen in hot water 14.1 
extract, 12.6 TWh S5 tee BGR itis Nie, RRP ES SOS os 
Hydrochlori id-in- 
os —— in 1.41 wat 1.41 at xe 
uble humin nitro- 1.87 1.27 
=. Humin nitrogen solu- 
ble in hydrochloric 2. 90 2.90 4.87 
acid. 
15. 50 
Amide nitrogen --.--- 15.20 15.35 25.72 
7.00 
Arginine nitrogen... 70 7.00 | 11.73 
Nitrogen soluble in 61.14 
hydrochloric acid. 55. 52 } 58.33 | Histidine nitrogen -._ 2.35 2.35 8,94 
: Lysine nitrogen -._--- 1.31 1,31 2.19 
4.05 93 
Cystine nitrogen-.___- 8. 82 8. 6.58 
25. 52 |] 
i 25. 04 
Mono-amino nitrogen_ 24,57 || 41. 96 
Non-amino nitrogen- 1.24 1.24 2.08 
Total nitrogen | 73.02 gc a coat er ee. 60.46 101.31 
=. a 
* The distribution of this nitrogen was not determined. 
Hydrochloric acid insoluble humin nitrogen (mg.) 1.84 
ydrochloric acid soluble nitrogen (mg.) 58.33 
Ni Total nitrogen insoluble in hot water (mg.) 59.67 
itrogen in hot water extract (mg.) 13.85 
Total nitrogen by direct analysis (meg. ) 73.02 
Total nitrogen accounted for (per cent) 99.75 
