186 Philippine Journal of Science 1920 
was proven not to be the case, as shown by the difference in their 
protein distribution. The results with the 100-mesh sample were 
still low. The method was then slightly modified, using the 
sample employed in the second trial. The mixture of sample 
and solvent was shaken repeatedly in a shaking machine, filtered 
and washed until the washing no longer gave a reaction for 
protein. Twelve hours were found to be sufficient to finish the 
extraction. This trial, although decidedly better than the first 
two, extracted only 47.55 per cent of the total protein. Since 
this laboratory has no facilities for very fine grinding, it was 
decided to discontinue the work for the present, and to report 
the results obtained. 
A comparison of the results of trials 1 and 2 shows that the 
two samples, one composed of the materials passed through the 
40- to 100-mesh sieves, and the other composed of the portion 
passed through the 100-mesh sieve, were not the same in general 
composition. The difference in protein distribution may be due 
to the fact that the sample used in trial 2 was composed of that 
portion of the copra meal which could be pulverized more easily; 
and it is not surprising to find, as in this case, that its 
composition was different from that of the portion harder to 
pulverize, 
The results of trial 3 show that the modified method used is 
better for copra meal than the one proposed by H. H. Snyder. 
In this trial, the percentage of salt-soluble protein is lower than 
in trial 2. Most of the water-soluble protein is also salt-soluble; 
and, therefore, an increase in the water-soluble extract naturally 
causes a decrease in the salt-soluble fraction. When the Per 
centages of the water- and the salt-soluble moieties are cO™ 
bined, the results in trial 3 show an improvement as compa 
with those in tria] 2 
a pea In percentage of protein that went into ie 
4 ae srs yielded more water- and gsalt-soluble than di 
passe pas sample, The water- plus the salt-soluble in a 
paREH coe a im trial 2 by about 5 per cent in the alkali-solub 
on . e former, which might explain the difference. 
a Practically no alcohol-soluble protein. This P4 
S with the observation of Osborne(13) that alcohol-soluble 
protein is not found in any seed but cereals. 
All the trials show th : saath most 
abundant in copra Coy the alkali-soluble protein is the 
Sap this work is incomplete, it is believed that it mY 
guide in the study of the biochemistry of copra ™°*" 
