590 Philippine Journal of Sctence 1920 
deviations from this standard are negligible if only small quan- 
tities of glucose are involved in the analysis; but they become 
of great importance when relatively large amounts are present 
in the solution. 
Experiments show that the mercury thread begins to fluctuate 
approximately thirty seconds after the thermometer reaches 95°. 
The temperature at the end of the thirty seconds, and just when 
Milligrams of glucose, 
10 zo 
, 40 se ks RE ee eS 
8 ! 
& 4 mn fr 
| i" 
: | i Ag ee is ee! 
e Ag pas] 
S30 ee | ne ree ea 
: Wha pon eee | ae ee 
z WIA | A iis ious oe H se) sae Ae) as om Sa Foe 
a, BS Beer 
: aa ey, Bc L = 3\¢ | ees fe eek Bee Sx pease Saat a 
x "c, G Oetak 3 ( oe RR Maras eg Pee Wa es ee 
= 1g¥// ae J Tas Se “eh ee pee ff ai 
2 Pee era 
2 . ] foot ‘aormes Sagem: cage Panos oleae Oo cared (aes AERA: in 
2 fi) Ro] | See 
Ss) }$—_t-—— + mee: sear, Tih S ee Se 
/ | | | | 
t oles FIO as, eae he 
ry | | 
| Bee ene. 
| Gok See 
a a | ag ren are ete SR Re ae ee 
bEBe Wins Gane ee Feat 
| ee ae | 
vas | | aa ee 
Fic. 5. Relation between milligrams of glucose and cubic centimeters of 0.05 N potassium 
permanganate solution reduced. Curves B are for 0.1 N and curves C, for 0.05 N potas- 
sium permanganate. Curve 1 is for 0.8 N alkali solution; curve 2, for 0.6 N solution; 
curve 8, for 0.4 N solution; curve 4, for 0.2 N solution; curve 5, for 0.04 N solution. 
fluctuation begins, has been observed to be about 104° to 
105° C. when the dilution is 100 cubic centimeters, and 110° C. 
when the dilution is 60 cubic centimeters. Boiling of the solu- 
tion starts within 5° before fluctuation begins. At this point, 
the rise of temperature is in most cases so rapid that it is impos- 
sible to time it accurately. 
THE INFLUENCE OF TIME AND TEMPERATURE UPON THE OXIDATION 
_ A series of experiments was performed to determine the 
influence of temperature and time of heating upon the degree 
