A new Volutella 



By Judson I'". Ci.AKK 



(Plate 371) 



This fungus was found growing on dead leaves of Paiidaniis 

 VeitcJiii in the greenhouses of the Botanical Department, Cornell 

 University. Pure cultures were obtained by the ordinary dilution 

 method, and germination and developmental phenomona were 

 studied by growing the fungus in hanging drop cultures in Van 



Tieghem cells. 



An excellent medium for the development of this form was 

 made by steeping 450 grams of sugar beet, sliced thin, in a liter of 

 water for three hours at 100° C. After straining and coolin<r, the 



whites of two eggs were added, and the infusion was again boiled, 

 then strained and filtered. To half the liter was added 6 grams of 

 agar for a solid medium. This infusion of sugar beet and its cor- 



responding agar were used with great satisfaction for the develop- 

 ment of various fungi, and were found to be particularly well 

 adapted for the development of many saprophytic forms. 



Hanging-drops of the infusion and the agar were inoculated with 

 a few spores from a pure culture. At 6 hrs. (Temp. 28^ C.) the 

 spores were germinating freely. The germ tubes were invariably 



developed from the 



/ 



the first stages appeared to be simply a bulging out of the hya- 

 line wall at these points. Two hours later the germ tubes had 

 reached a length of about 20// and were beginning to branch by 

 developing a branch close to the end of the original conidium, 

 which could still be distinguished from the germ tubes by its greater 



diameter. 



(/• 



constant in all cases observed. At 15 hours the cultures presented 

 a mass of well branched, vigorously growing, non-septate my- 

 celium. 



At from 24 to 36 hours conidial fructifications of two distinct 

 types made their appearance. The first to appear were the larger 

 submerged sporophores bearing macroconidia. In origin, mode 



(()17) 



