CIS Clark : A new Volutella 



of development, and appearance they resembled the aerial sporo- 

 phores developed later, but differed from them In size, submerged 

 habit, and character and number of conidia borne. In Fig. 5 is 

 shown a branch of mycelium bearing the macrosporophores, two of 

 which have developed conidia. This drawing was made from a 

 culture in agar where the conidia were held i/i sifn by the medium 

 and could be counted. In general each macrosporophorc pro- 

 duced 8-12 macroconidia. These latter were rather irregular In 

 shape, varied greatly in size, and were obscurely two-guttate in ap- 

 pearance. The measurements varied from 3.5 /-^X 7 /^ to 4.5 nx 18 /^. 

 Several of these macroconidia are shown in Fig. 2. In germina- 

 tion phenomena they were quite similar to the microconidia. 



Some hours after the first appearance of the macrosporophores, 

 smaller, aerial microsporophores were very abundantly developed. 

 These were borne laterally on submerged, and laterally and termi- 

 nally on aerial hyphac, and abstricted conidia from their apices 

 exactly similar to those examined from the original sporodochia 



on leaves of PiXJidanus. In figures 6-9 the manner of develop- 

 ment of these conidia is shown, and how^ they remain clustered at 

 the apex of the sporophore^ held in position by capillary moisture 

 forming a mucro-llkc aggregation wdiich sometimes contained a 

 hundred or more microconidia. 



On the tenth day sporodochia were observed in the agar cul- 

 tures. The earliest stage observed was the development of a 

 number of sporophorcs, in close proximity, bearing a large aggre- 

 gation of conidia showing in mass a light honey color. Later, the 

 characteristic setae began to make their appearance. Originating 

 in the mass of hyphac near or at the base of the sporodochium, 

 they passed outwards and upwards at varj-ing angles emerging 

 through the spore masses at varying points. The mature sporo- 

 dochia in these cultures resembled very closely those originally 

 found on PandiX}nis,\^\\X. differed in having a somewhat more regu- 

 lar appearance and a richer yellow color, variations due no doubt 



to the altered conditions of development. 



On sterile bean pods and sugar beet plugs the growth and 



conidial fructification was excellent, and quite similar to that already 

 described for the sugar beet infusion and agar. No indication of 

 a perfect (asciis) stage was obser\^ed. 



