84 
There also some directions for fixing the protoplasm, and the 
following solution is recommended: 
Hydrarg. bi. chlor. 65 gms. 
Common salt, 15 gms. 
Water to make, 100 c. c. 
Specific gravity should be about 1.62. 
This solution fixes the protoplasm almost instantaneously, and 
the coloring methods can then be applied as before. The details 
of the nucleus are given and figured for Coscinodiscus concinnus. 
In conclusion the author states: “I have said that one could ren- 
der prominent by the use of methylene blue the chromatine of 
the nucleus of the diatoms. I recall that this nucleus is alwayS 
immersed in a special pinnuclear protoplasm, the properties and i 
use of which still remains to be studied; that this protoplasm 5 — 
always placed in large masses in the centre of the figure of the . 
diatoms; that habitually it forms a bridge, going from one valve e 
to the nS that it is in this protoplasm that one observes the * 
movements of the nucleus, its division or its fragmentation. 
S. EJ 
Flore Bryologique du Japon, Nouveux Documents. Emile Besche 
relle (Ann. Sci. Nat. (VIL) 17: 327-393, 1893). _ 
The collections enumerated in this paper were made by thé = 
Abbé Faurie on the islands of Nippon and Yezo, and some of ie 
Savatier’s from the vicinity of Yokohama are included. They 
supplement the enumeration given by Mitten in 1891, and add 
many new species to the flora. Many of them bore manu 
script names given by Schimper, and as far as possible these hav® 
been used, but the larger part of the collections, made by M. 
Faurie, in 1885-1891, are entirely new and from a hitherto unex” 
plored part of Japan. Considering the similarity of the moss 
flora of our Western States with that of Japan, it will be well 0. 
make some comparisons of the new species, with the new ones bet 
recently published by Kindberg, and the monographers of the 
Roll collection. There may be some duplication of names, eve? 
here. = . E. G. ee 
