350 
Proceedings of the Botanical Club, A. A. A. S., Buffalo Meeting, 
August 25-29, 1896. 
Tuespay, AUGUST 25TH. MORNING. 
In the absence of Mr. Coville, President, and Prof. MacMillan, 
Vice-President, Prof. Kellerman was elected President pro “em. 
Mr. Cowell, Secretary, submitted the registration book for signa- 
tures of members present and distributed badges. 
Prof. Kellerman distributed copies of a map of Ohio, showing 
the distribution in the southern counties of that state of Phoraden- 
dvon flavescens, Bignonia crucigera, which covers the fences in some 
places, and Polypodium polypodioides. Prof. Coulter remarked on 
the distribution of the Phoradendron in the lower Wabash region 
of Indiana and Illinois. Prof. Tracy stated that it is abundant in 
southern Illinois, and Prof. MacDougal recorded its occurrence at 
points in southern Indiana. 
Prof. Kellerman remarked that the Canada Thistle, Cavduus 
avvensis does not spread in southern Ohio. Prof. Lazenby stated 
that seeds of the plant are not formed in the region. 
Prof. L. R. Jones described a method of distributing pure cul- 
tures of fungi. Owing to mixed growth of several species of fungi 
upon the same substratum, it is often difficult to obtain an uncon- 
taminated growth for distribution in exsiccati. The growth and 
distribution of pure cultures easily obviates this difficulty. These 
pure cultures can readily be made in quantity and in convenient 
form for distribution by placing paraffined paper in the bottom of 
large culture dishes; the agar, or other nutrient medium, is then 
poured upon this, and seeded with spores from a pure culture. 
When colonies have reached sufficient development, the whole is 
dried down, the paper bearing the fungus is removed from the 
dish and cut up for distribution. Prof. Jones suggested that the 
method might be applied to the distribution of bacterial cultures. 
Concerning this, Prof. Russell stated that the method could 
not be used for the propagation of such cultures, as the bac- 
teria, especially the non-sporogenous forms, die out so easily 
when subjected to desiccation. It might be of service, however, 
in preserving the appearance of colonies. Mr. Duggar inquired if 
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