THE SPERMATOGENESIS OF Hysrip PEAS §29 
is an especial preparation for synapsis, the condition of the outer 
one is surely paradoxical. 
The nucleus of the spore mother-cell enters into a complete 
resting condition in which it remains during much or most of the 
period of growth of the cell. As a preparation for synapsis the 
chromatin of the recticulum collects into masses of irregular shape 
and size, more or less arranged in threads, about the periphery of 
the nuclear vacuole. The chromatin-thread at length takes up a 
position around or closely applied to the nucleolus and the synap- 
sis stage results. When the mass of chromatin unloosens, it is 
seen to be made up of a much bent ribbon. The spireme is at first 
beaded in appearance, but it becomes more regular in outline and 
also increases in its capacity for absorbing stains. The splitting of 
the spireme was not clearly seen, although evidences of it were no- 
ticed. Ina few cells I saw the chromatin-loops which are char- 
acteristic of the prophases of the first maturation mitosis (/, 75-77), 
but in each case the loop was not contracted and did not show 
the second fission which was observed in the cotton hybrid. 
The two maturation divisions were quite like those seen in Ser- 
pette and in Fillbasket x Debarbieux. The reduced number of 
chromosomes was seven. 
A difference in the origin of the multipolar spindle of the first 
mitosis of Express x Serpette from that of Fillbasket x Debar- 
bieux was noted (this stage was not seen in Serpette), but whether 
it was an artefact or not I was unable, because of the lack of ma- 
terial, definitely to determine. In several spore mother-cells the 
cytoplasm was very dense in the immediate neighborhood of the 
nucleus, forming what was in effect a perinuclear zone. In these 
cells the multipolar spindle seemed to have its origin wholly within 
the nucleus. The linin became lumpy and very distinct, it formed 
a mesh-work which gave the appearance of polarity, and had every 
indication of being the genesis of the multipolar spindle. The 
nuclear wall remained in some cases recognizable even when the 
intranuclear material was of a pronounced fibrous character. The 
departure from the extranuclear origin of the spindle observed in 
Fillbasket x Debarbieux is certainly a marked one, and interesting 
€ven if it proves in the end to be an artefact. 
